Abstract
We studied the distribution of genes and recombination in wheat (Triticum aestivum) group 1 chromosomes by comparing high-density physical and genetic maps. Physical maps of chromosomes 1A, 1B, and 1D were generated by mapping 50 DNA markers on 56 single-break deletion lines. A consensus physical map was compared with the 1D genetic map of Triticum tauschii (68 markers) and a Triticeae group 1 consensus map (288 markers) to generate a cytogenetic ladder map (CLM). Most group 1 markers (86%) were present in five clusters that encompassed only 10% of the group 1 chromosome. This distribution may reflect that of genes because more than half of the probes were cDNA clones and 30% were PstI genomic. All 14 agronomically important genes in group 1 chromosomes were present in these clusters. Most recombination occurred in gene-cluster regions. Markers fell at an average distance of 244 kb in these regions. The CLM involving the Triticeae consensus genetic map revealed that the above distribution of genes and recombination is the same in other Triticeae species. Because of a significant number of common markers, our CLM can be used for comparative mapping and to estimate physical distances among markers in many Poaceae species including rice and maize.
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- Ahn S., Anderson J. A., Sorrells M. E., Tanksley S. D. Homoeologous relationships of rice, wheat and maize chromosomes. Mol Gen Genet. 1993 Dec;241(5-6):483–490. doi: 10.1007/BF00279889. [DOI] [PubMed] [Google Scholar]
- Ahn S., Tanksley S. D. Comparative linkage maps of the rice and maize genomes. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):7980–7984. doi: 10.1073/pnas.90.17.7980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bennetzen J. L., Freeling M. Grasses as a single genetic system: genome composition, collinearity and compatibility. Trends Genet. 1993 Aug;9(8):259–261. doi: 10.1016/0168-9525(93)90001-x. [DOI] [PubMed] [Google Scholar]
- Bollag R. J., Waldman A. S., Liskay R. M. Homologous recombination in mammalian cells. Annu Rev Genet. 1989;23:199–225. doi: 10.1146/annurev.ge.23.120189.001215. [DOI] [PubMed] [Google Scholar]
- Gill K. S., Gill B. S., Endo T. R., Boyko E. V. Identification and high-density mapping of gene-rich regions in chromosome group 5 of wheat. Genetics. 1996 Jun;143(2):1001–1012. doi: 10.1093/genetics/143.2.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hohmann U., Endo T. R., Gill K. S., Gill B. S. Comparison of genetic and physical maps of group 7 chromosomes from Triticum aestivum L. Mol Gen Genet. 1994 Dec 1;245(5):644–653. doi: 10.1007/BF00282228. [DOI] [PubMed] [Google Scholar]
- Kurata N., Nagamura Y., Yamamoto K., Harushima Y., Sue N., Wu J., Antonio B. A., Shomura A., Shimizu T., Lin S. Y. A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nat Genet. 1994 Dec;8(4):365–372. doi: 10.1038/ng1294-365. [DOI] [PubMed] [Google Scholar]
- Lichten M., Goldman A. S. Meiotic recombination hotspots. Annu Rev Genet. 1995;29:423–444. doi: 10.1146/annurev.ge.29.120195.002231. [DOI] [PubMed] [Google Scholar]
- Liu Y. G., Tsunewaki K. Restriction fragment length polymorphism (RFLP) analysis in wheat. II. Linkage maps of the RFLP sites in common wheat. Jpn J Genet. 1991 Oct;66(5):617–633. doi: 10.1266/jjg.66.617. [DOI] [PubMed] [Google Scholar]
- Nilsson N. O., Säll T., Bengtsson B. O. Chiasma and recombination data in plants: are they compatible? Trends Genet. 1993 Oct;9(10):344–348. doi: 10.1016/0168-9525(93)90038-j. [DOI] [PubMed] [Google Scholar]
- Werner J. E., Endo T. R., Gill B. S. Toward a cytogenetically based physical map of the wheat genome. Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11307–11311. doi: 10.1073/pnas.89.23.11307. [DOI] [PMC free article] [PubMed] [Google Scholar]