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. 1997 Feb;145(2):243–251. doi: 10.1093/genetics/145.2.243

Interaction between Ustilago Maydis Rec2 and Rad51 Genes in DNA Repair and Mitotic Recombination

D O Ferguson 1, M C Rice 1, M H Rendi 1, H Kotani 1, E B Kmiec 1, W K Holloman 1
PMCID: PMC1207791  PMID: 9071580

Abstract

A gene encoding a Ustilago maydis Rad51 orthologue has been isolated. rad51-1, a mutant constructed by disrupting the gene, was as sensitive to killing by ultraviolet light and γ radiation as the rec2-1 mutant and slightly more sensitive to killing by methyl methanesulfonate. There was no suppression of killing by ultraviolet light when a rec2-1 strain was transformed with a multicopy plasmid containing RAD51, nor was there suppression when rad51-1 was transformed with a multicopy plasmid containing REC2. Recombination proficiency as measured by a gap repair assay was diminished in both rec2-1 and rad51-1 strains. In rec2-1 the frequency of recombination was decreased, but the spectrum of events was similar to that observed in wild type, while in rad51-1 the frequency as well as the spectrum of recombination events were different. Studies with the rec2-1 rad51-1 double mutant indicated that there was epistasis in the action of REC2 and RAD51 in certain repair and recombination functions, but some measure of independent action in other functions.

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Selected References

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  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Bai Y., Symington L. S. A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae. Genes Dev. 1996 Aug 15;10(16):2025–2037. doi: 10.1101/gad.10.16.2025. [DOI] [PubMed] [Google Scholar]
  3. Basile G., Aker M., Mortimer R. K. Nucleotide sequence and transcriptional regulation of the yeast recombinational repair gene RAD51. Mol Cell Biol. 1992 Jul;12(7):3235–3246. doi: 10.1128/mcb.12.7.3235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bauchwitz R., Holloman W. K. Isolation of the REC2 gene controlling recombination in Ustilago maydis. Gene. 1990 Dec 15;96(2):285–288. doi: 10.1016/0378-1119(90)90265-s. [DOI] [PubMed] [Google Scholar]
  5. Dunderdale H. J., West S. C. Recombination genes and proteins. Curr Opin Genet Dev. 1994 Apr;4(2):221–228. doi: 10.1016/s0959-437x(05)80048-6. [DOI] [PubMed] [Google Scholar]
  6. Ferguson D. O., Holloman W. K. Recombinational repair of gaps in DNA is asymmetric in Ustilago maydis and can be explained by a migrating D-loop model. Proc Natl Acad Sci U S A. 1996 May 28;93(11):5419–5424. doi: 10.1073/pnas.93.11.5419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fotheringham S., Holloman W. K. Cloning and disruption of Ustilago maydis genes. Mol Cell Biol. 1989 Sep;9(9):4052–4055. doi: 10.1128/mcb.9.9.4052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fotheringham S., Holloman W. K. Extrachromosomal recombination is deranged in the rec2 mutant of Ustilago maydis. Genetics. 1991 Dec;129(4):1052–1060. [PMC free article] [PubMed] [Google Scholar]
  9. Game J. C., Cox B. S. Epistatic interactions between four rad loci in yeast. Mutat Res. 1972 Dec;16(4):353–362. doi: 10.1016/0027-5107(72)90203-5. [DOI] [PubMed] [Google Scholar]
  10. Game J. C. DNA double-strand breaks and the RAD50-RAD57 genes in Saccharomyces. Semin Cancer Biol. 1993 Apr;4(2):73–83. [PubMed] [Google Scholar]
  11. Gerhold D., Thiyagarajan M., Kmiec E. B. The topoisomerase I gene from Ustilago maydis: sequence, disruption and mutant phenotype. Nucleic Acids Res. 1994 Sep 11;22(18):3773–3778. doi: 10.1093/nar/22.18.3773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hays S. L., Firmenich A. A., Berg P. Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins. Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6925–6929. doi: 10.1073/pnas.92.15.6925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holliday R. Altered recombination frequencies in radiation sensitivie strains of Ustilago. Mutat Res. 1967 May-Jun;4(3):275–288. doi: 10.1016/0027-5107(67)90022-x. [DOI] [PubMed] [Google Scholar]
  14. Johnson R. D., Symington L. S. Functional differences and interactions among the putative RecA homologs Rad51, Rad55, and Rad57. Mol Cell Biol. 1995 Sep;15(9):4843–4850. doi: 10.1128/mcb.15.9.4843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kans J. A., Mortimer R. K. Nucleotide sequence of the RAD57 gene of Saccharomyces cerevisiae. Gene. 1991 Aug 30;105(1):139–140. doi: 10.1016/0378-1119(91)90527-i. [DOI] [PubMed] [Google Scholar]
  16. Kmiec E. B., Cole A., Holloman W. K. The REC2 gene encodes the homologous pairing protein of Ustilago maydis. Mol Cell Biol. 1994 Nov;14(11):7163–7172. doi: 10.1128/mcb.14.11.7163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kmiec E., Holloman W. K. Homologous pairing of DNA molecules promoted by a protein from Ustilago. Cell. 1982 Jun;29(2):367–374. doi: 10.1016/0092-8674(82)90153-2. [DOI] [PubMed] [Google Scholar]
  18. Lovett S. T. Sequence of the RAD55 gene of Saccharomyces cerevisiae: similarity of RAD55 to prokaryotic RecA and other RecA-like proteins. Gene. 1994 May 3;142(1):103–106. doi: 10.1016/0378-1119(94)90362-x. [DOI] [PubMed] [Google Scholar]
  19. McDonald J. P., Rothstein R. Unrepaired heteroduplex DNA in Saccharomyces cerevisiae is decreased in RAD1 RAD52-independent recombination. Genetics. 1994 Jun;137(2):393–405. doi: 10.1093/genetics/137.2.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Morrison D. P., Hastings P. J. Characterization of the mutator mutation mut5-1. Mol Gen Genet. 1979 Aug;175(1):57–65. doi: 10.1007/BF00267856. [DOI] [PubMed] [Google Scholar]
  21. Muris D. F., Vreeken K., Carr A. M., Broughton B. C., Lehmann A. R., Lohman P. H., Pastink A. Cloning the RAD51 homologue of Schizosaccharomyces pombe. Nucleic Acids Res. 1993 Sep 25;21(19):4586–4591. doi: 10.1093/nar/21.19.4586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Onel K., Thelen M. P., Ferguson D. O., Bennett R. L., Holloman W. K. Mutation avoidance and DNA repair proficiency in Ustilago maydis are differentially lost with progressive truncation of the REC1 gene product. Mol Cell Biol. 1995 Oct;15(10):5329–5338. doi: 10.1128/mcb.15.10.5329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Orr-Weaver T. L., Szostak J. W., Rothstein R. J. Yeast transformation: a model system for the study of recombination. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354–6358. doi: 10.1073/pnas.78.10.6354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rattray A. J., Symington L. S. Use of a chromosomal inverted repeat to demonstrate that the RAD51 and RAD52 genes of Saccharomyces cerevisiae have different roles in mitotic recombination. Genetics. 1994 Nov;138(3):587–595. doi: 10.1093/genetics/138.3.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rubin B. P., Li D., Holloman W. K. The LEU1 gene of Ustilago maydis. Gene. 1994 Mar 11;140(1):131–135. doi: 10.1016/0378-1119(94)90743-9. [DOI] [PubMed] [Google Scholar]
  26. Shinohara A., Ogawa H., Matsuda Y., Ushio N., Ikeo K., Ogawa T. Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat Genet. 1993 Jul;4(3):239–243. doi: 10.1038/ng0793-239. [DOI] [PubMed] [Google Scholar]
  27. Story R. M., Weber I. T., Steitz T. A. The structure of the E. coli recA protein monomer and polymer. Nature. 1992 Jan 23;355(6358):318–325. doi: 10.1038/355318a0. [DOI] [PubMed] [Google Scholar]
  28. Sugawara N., Ivanov E. L., Fishman-Lobell J., Ray B. L., Wu X., Haber J. E. DNA structure-dependent requirements for yeast RAD genes in gene conversion. Nature. 1995 Jan 5;373(6509):84–86. doi: 10.1038/373084a0. [DOI] [PubMed] [Google Scholar]
  29. Tsukuda T., Carleton S., Fotheringham S., Holloman W. K. Isolation and characterization of an autonomously replicating sequence from Ustilago maydis. Mol Cell Biol. 1988 Sep;8(9):3703–3709. doi: 10.1128/mcb.8.9.3703. [DOI] [PMC free article] [PubMed] [Google Scholar]

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