Abstract
Iberian minnows collectively known as the Tropidophoxinellus alburnoides STEINDACHNER complex comprise diploid and polyploid forms with highly female biased sex ratios. Previous investigators suggested that all-female clonal reproduction and interspecific hybridization may occur in this complex. We examined nuclear (allozymes) and cytoplasmic genes (mtDNA) to assess the evolutionary origins, relationships, and reproductive modes of T. alburnoides from western Spain. The multi-locus allozyme data clearly revealed the hybrid nature of all polyploid forms of this fish and some diploid forms as well. Diagnostic markers identified fish from the genus Leuciscus as the paternal ancestor of hybrids in the Duero and Guadiana River Basins. Additionally, analysis of nuclear markers revealed that hybridogenetic reproduction occurs in the diploid and triploid hybrids. The hybrids fully express the paternal Leuciscus genome and then discard it during oogenesis. Hybridogenetic ova contain only maternal nuclear genes and mtDNA from a non-hybrid T. alburnoides ancestor. Apparently diploid and triploid hybrids of T. alburnoides persist as sperm parasites on males of a sexually reproducing Leuciscus host species.
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- Brown W. M., Wright J. W. Mitochondrial DNA analyses and the origin and relative age of parthenogenetic lizards (genus Cnemidophorus). Science. 1979 Mar 23;203(4386):1247–1249. doi: 10.1126/science.424751. [DOI] [PubMed] [Google Scholar]
- Leslie J. F., Vrijenhoek R. C. Genetic dissection of clonally inherited genomes of poeciliopsis. I. Linkage analysis and preliminary assessment of deleterious gene loads. Genetics. 1978 Dec;90(4):801–811. doi: 10.1093/genetics/90.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McElroy D., Moran P., Bermingham E., Kornfield I. REAP: an integrated environment for the manipulation and phylogenic analysis of restriction data. J Hered. 1992 Mar-Apr;83(2):157–158. doi: 10.1093/oxfordjournals.jhered.a111180. [DOI] [PubMed] [Google Scholar]
- Nei M., Li W. H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269–5273. doi: 10.1073/pnas.76.10.5269. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quattro J. M., Avise J. C., Vrijenhoek R. C. An ancient clonal lineage in the fish genus Poeciliopsis (Atheriniformes: Poeciliidae). Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):348–352. doi: 10.1073/pnas.89.1.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quattro J. M., Avise J. C., Vrijenhoek R. C. Mode of origin and sources of genotypic diversity in triploid gynogenetic fish clones (Poeciliopsis: Poeciliidae). Genetics. 1992 Mar;130(3):621–628. doi: 10.1093/genetics/130.3.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quattro J. M., Avise J. C., Vrijenhoek R. C. Molecular evidence for multiple origins of hybridogenetic fish clones (Poeciliidae:Poeciliopsis). Genetics. 1991 Feb;127(2):391–398. doi: 10.1093/genetics/127.2.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vindeløv L. L., Christensen I. J., Keiding N., Spang-Thomsen M., Nissen N. I. Long-term storage of samples for flow cytometric DNA analysis. Cytometry. 1983 Mar;3(5):317–322. doi: 10.1002/cyto.990030502. [DOI] [PubMed] [Google Scholar]