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. 1997 Oct;147(2):589–596. doi: 10.1093/genetics/147.2.589

Homology-Dependent Silencing of the Sc3 Gene in Schizophyllum Commune

T A Schuurs 1, EAM Schaeffer 1, JGH Wessels 1
PMCID: PMC1208181  PMID: 9335596

Abstract

After introduction of extra copies of the SC3 hydrophobin gene into a wild-type strain of Schizophyllum commune, gene silencing was observed acting on both endogenous and introduced SC3 genes in primary vegetative transformants. Nuclear run-on experiments indicated that silencing acted at the transcriptional level. Southern analysis revealed that cytosine methylation of genomic DNA occurred. Moreover, SC3 silencing was suppressed by exposure to 5-azacytidine during growth. After growth of SC3-suppressed colonies from homogenized mycelium or from colonies stored at 4°, SC3 transcription was restored. However, after prolonged growth SC3 silencing was again observed. Introduction of a promoterless SC3 fragment into wild type gave less SC3 silencing.

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Selected References

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