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. 1997 Dec;147(4):1769–1782. doi: 10.1093/genetics/147.4.1769

The Accumulation of P-Element-Induced Recombinants in the Germline of Male Drosophila Melanogaster

M M Tanaka 1, X M Liang 1, YHM Gray 1, J A Sved 1
PMCID: PMC1208345  PMID: 9409835

Abstract

P-element-induced recombination in Drosophila melanogaster occurs premeiotically. Recombinants are therefore expected to accumulate in the stem cells of the germline of P-element-carrying males. We show that both the recombination frequency and the incidence of ``clustering'' increase with the age of males carrying various P-element derivatives. The combination of end-deleted elements can lead to average recombination frequencies >50% with individual instances of 100% recombination. These elements also lowered the fertility of the carriers. We investigated these features by constructing an analytical and a computer simulation model of the course of events in the germline, incorporating the recently proposed hybrid element insertion (HEI) model of P-element activity. The model is able to predict extreme recombination levels, segregation ratio biases and lowered fertility through cell death in a single analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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