Abstract
DNA amounts in macronuclei and micronuclei of Tetrahymena pyriformis were measured by Feulgen microspectrophotometry. Assuming that the unreplicated micronucleus is diploid, the unreplicated macronucleus was found to contain approximately 45 times the haploid DNA amount. The relationship of these findings to the 45 independently assorting genetic subunits characterized by Allen and Nanney and their collaborators is discussed. The pattern of DNA synthesis in macro- and micronuclei during the cell cycle is also described.
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Selected References
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- Ammermann D. Morphology and development of the macronuclei of the ciliates Stylonychia mytilus and Euplotes aediculatus. Chromosoma. 1971;33(2):209–238. doi: 10.1007/BF00285634. [DOI] [PubMed] [Google Scholar]
- Bleyman L. K., Simon E. M., Brosi R. Sequential nuclear differentiation in Tetrahymena. Genetics. 1966 Jul;54(1):277–291. doi: 10.1093/genetics/54.1.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bleyman L. K., Simon E. M. Clonal analysis of nuclear differentiation in Tetrahymena. Dev Biol. 1968 Sep;18(3):217–231. doi: 10.1016/0012-1606(68)90033-x. [DOI] [PubMed] [Google Scholar]
- ORNSTEIN L. The distributional error in microspectrophotometry. Lab Invest. 1952;1(2):250–265. [PubMed] [Google Scholar]