Abstract
A fraction of recombinant colonies resulting from conjugation is heterogenetic for unselected markers. Constitutivity for alkaline phosphatase synthesis (phoR) is studied as the unselected marker. The frequency of phoR heterogeneity depends on the genetic distance between phoR and the selected marker. Various models are considered which explain the formation of heterogenetic colonies (mixed clones), and experiments are described which test these models. It is concluded that the Hfr fragment can replicate and participate more than once in recombination thus yielding heterogenetic colonies.
Full Text
The Full Text of this article is available as a PDF (794.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ECHOLS H., GAREN A., GAREN S., TORRIANI A. Genetic control of repression of alkaline phosphatase in E. coli. J Mol Biol. 1961 Aug;3:425–438. doi: 10.1016/s0022-2836(61)80055-7. [DOI] [PubMed] [Google Scholar]
- Gross J. D., Caro L. G. DNA transfer in bacterial conjugation. J Mol Biol. 1966 Apr;16(2):269–284. doi: 10.1016/s0022-2836(66)80172-9. [DOI] [PubMed] [Google Scholar]
- Itoh T., Tomizawa J. Inactivation of chromosomal fragments transferred from hfr strains. Genetics. 1971 May;68(1):1–11. doi: 10.1093/genetics/68.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ohki M., Tomizawa J. Asymmetric transfer of DNA strands in bacterial conjugation. Cold Spring Harb Symp Quant Biol. 1968;33:651–658. doi: 10.1101/sqb.1968.033.01.074. [DOI] [PubMed] [Google Scholar]
- Rosner A., Yagil E. Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants. Mol Gen Genet. 1970;106(3):254–262. doi: 10.1007/BF00340384. [DOI] [PubMed] [Google Scholar]
- Whitehouse H. L. The mechanism of genetic recombination. Biol Rev Camb Philos Soc. 1970 May;45(2):265–315. doi: 10.1111/j.1469-185x.1970.tb01633.x. [DOI] [PubMed] [Google Scholar]
- Wilkins B. M., Hollom S. E., Rupp W. D. Deoxyribonucleic acid transferred from ultraviolet-irradiated excision-defective Hfr cells of Escherichia coli K-12. J Bacteriol. 1971 Aug;107(2):505–512. doi: 10.1128/jb.107.2.505-512.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wood T. H. Genetic recombination in Escherichia coli: clone heterogeneity and the kinetics of segregation. Science. 1967 Jul 21;157(3786):319–321. doi: 10.1126/science.157.3786.319. [DOI] [PubMed] [Google Scholar]