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. 1972 Nov;72(3):393–410. doi: 10.1093/genetics/72.3.393

Genetic Map of the Lactose Repressor Gene (i) of ESCHERICHIA COLI

Magnus Pfahl 1
PMCID: PMC1212838  PMID: 4566215

Abstract

39 tonB i- deletions were used to map 57 independently isolated i gene mutants. Methods selective for i+ recombinants from various types of i mutants are described. i+ recombination frequencies of 6 x 10-6 to 6 x 10-7 can be detected in the different selective systems. Twenty is mutations and 12 i-d mutations map in distinct but different regions of the gene. i-sus mutations are scattered over the gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler K., Beyreuther K., Fanning E., Geisler N., Gronenborn B., Klemm A., Müller-Hill B., Pfahl M., Schmitz A. How lac repressor binds to DNA. Nature. 1972 Jun 9;237(5354):322–327. doi: 10.1038/237322a0. [DOI] [PubMed] [Google Scholar]
  2. COHN M., HORIBATA K. Analysis of the differentiation and of the heterogeneity within a population of Escherichia coli undergoing induced beta-galactosidase synthesis. J Bacteriol. 1959 Nov;78:613–623. doi: 10.1128/jb.78.5.613-623.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davies J., Jacob F. Genetic mapping of the regulator and operator genes of the lac operon. J Mol Biol. 1968 Sep 28;36(3):413–417. doi: 10.1016/0022-2836(68)90165-4. [DOI] [PubMed] [Google Scholar]
  4. FUKASAWA T., NIKAIDO H. Galactose-sensitive mutants of Salmonella. II. Bacteriolysis induced by galactose. Biochim Biophys Acta. 1961 Apr 15;48:470–483. doi: 10.1016/0006-3002(61)90045-2. [DOI] [PubMed] [Google Scholar]
  5. HORIUCHI T., NOVICK A. A thermolabile repression system. Cold Spring Harb Symp Quant Biol. 1961;26:247–248. doi: 10.1101/sqb.1961.026.01.030. [DOI] [PubMed] [Google Scholar]
  6. Ippen K., Miller J. H., Scaife J., Beckwith J. New controlling element in the Lac operon of E. coli. Nature. 1968 Mar 2;217(5131):825–827. doi: 10.1038/217825a0. [DOI] [PubMed] [Google Scholar]
  7. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  8. Jobe A., Riggs A. D., Bourgeois S. Lac repressor-operator interaction. V. Characterization of super- and pseudo-wild-type repressors. J Mol Biol. 1972 Feb 28;64(1):181–199. doi: 10.1016/0022-2836(72)90328-2. [DOI] [PubMed] [Google Scholar]
  9. Miller J. H., Beckwith J., Muller-Hill B. Direction of transcription of a regulatory gene in E. coli. Nature. 1968 Dec 28;220(5174):1287–1290. doi: 10.1038/2201287a0. [DOI] [PubMed] [Google Scholar]
  10. Miller J. H., Ippen K., Scaife J. G., Beckwith J. R. The promoter-operator region of the lac operon of Escherichia coli. J Mol Biol. 1968 Dec;38(3):413–420. doi: 10.1016/0022-2836(68)90395-1. [DOI] [PubMed] [Google Scholar]
  11. Miller J. H., Reznikoff W. S., Silverstone A. E., Ippen K., Signer E. R., Beckwith J. R. Fusions of the lac and trp Regions of the Escherichia coli Chromosome. J Bacteriol. 1970 Dec;104(3):1273–1279. doi: 10.1128/jb.104.3.1273-1279.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Myers G. L., Sadler J. R. Mutational inversion of control of the lactose operon of Escherichia coli. J Mol Biol. 1971 May 28;58(1):1–28. doi: 10.1016/0022-2836(71)90229-4. [DOI] [PubMed] [Google Scholar]
  13. Norkin L. C. Marker-specific effects in genetic recombination. J Mol Biol. 1970 Aug;51(3):633–655. doi: 10.1016/0022-2836(70)90013-6. [DOI] [PubMed] [Google Scholar]
  14. Novick A., Weiner M. ENZYME INDUCTION AS AN ALL-OR-NONE PHENOMENON. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):553–566. doi: 10.1073/pnas.43.7.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Platt T., Weber K., Ganem D., Miller J. H. Translational restarts: AUG reinitiation of a lac repressor fragment. Proc Natl Acad Sci U S A. 1972 Apr;69(4):897–901. doi: 10.1073/pnas.69.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reznikoff W. S., Miller J. H., Scaife J. G., Beckwith J. R. A mechanism for repressor action. J Mol Biol. 1969 Jul 14;43(1):201–213. doi: 10.1016/0022-2836(69)90089-8. [DOI] [PubMed] [Google Scholar]
  17. SADLER J. R., NOVICK A. THE PROPERTIES OF REPRESSOR AND THE KINETICS OF ITS ACTION. J Mol Biol. 1965 Jun;12:305–327. doi: 10.1016/s0022-2836(65)80255-8. [DOI] [PubMed] [Google Scholar]
  18. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yanofsky C., Drapeau G. R., Guest J. R., Carlton B. C. THE COMPLETE AMINO ACID SEQUENCE OF THE TRYPTOPHAN SYNTHETASE A PROTEIN (alpha SUBUNIT) AND ITS COLINEAR RELATIONSHIP WITH THE GENETIC MAP OF THE A GENE. Proc Natl Acad Sci U S A. 1967 Feb;57(2):296–298. doi: 10.1073/pnas.57.2.296. [DOI] [PMC free article] [PubMed] [Google Scholar]

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