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. 1973 Jul;74(3):521–531. doi: 10.1093/genetics/74.3.521

Gene Dosage and the Expression of Electrophoretic Patterns in Heteroploid Mouse Cell Lines

Rosann A Farber 1
PMCID: PMC1212966  PMID: 4795559

Abstract

Spontaneously transformed mouse cell lines heterozygous for electrophoretic markers have been studied to determine the relationship between gene dosage and phenotype. It is shown that a clone with an electrophoretic pattern for glucosephosphate isomerase of three bands in a ratio of 4A:4AB:1B contains three copies of chromosome 7, which carries the gene for this enzyme. A clone from a different line with a pattern of three bands in a ratio of 1A:6AB:9B for NADP-isocitrate dehydrogenase has four copies of the chromosome carrying this gene or three copies plus a rearrangement which apparently involves this chromosome. These results show that all of the alleles for each enzyme are expressed to an equal extent in these cells.

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Selected References

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