Skip to main content
NCBI home page
Genetics logoLink to Genetics
. 1973 Sep;75(1):35–48. doi: 10.1093/genetics/75.1.35

Integration Efficiency in DNA-Induced Transformation of PNEUMOCOCCUS. II. Genetic Studies of Mutant Integrating All the Markers with a High Efficiency

Gérard Tiraby 1, Michel Armand Sicard 1
PMCID: PMC1213000  PMID: 4148667

Abstract

Transformation of the pneumococcus mutant 401 by DNA's bearing the standard reference marker and several other markers belonging to two unlinked loci has shown that differences in the integration efficiencies of these markers were considerably reduced in this strain compared to the wild-type strain Cl3. The sensitivities of mutant 401 to ultraviolet light and to X-ray irradiation are the same as those of Cl3. However, in 401 all the markers tested are more resistant to inactivation as shown by transformation of 401 and Cl3 by ultraviolet-irradiated DNA. The increase in resistance is greater for low efficiency (LE) markers than for high efficiency (HE) markers.—The decreased discrimination between LE and HE markers in strain 401 is not due to a mechanism related to modification of markers in the transforming DNA by the recipient cells, nor are the proteins inducing competence of the cells responsible for the differences in the integration efficiencies of various markers.—Genetic studies of the fate of recombinants as well as the measure of the amount of DNA taken up have shown that all the markers are integrated in strain 401 by the same recombination process, that specific to high efficiency markers.

Full Text

The Full Text of this article is available as a PDF (768.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ephrussi-Taylor H., Sicard A. M., Kamen R. Genetic Recombination in DNA-Induced Transformation of Pneumococcus. I. the Problem of Relative Efficiency of Transforming Factors. Genetics. 1965 Mar;51(3):455–475. doi: 10.1093/genetics/51.3.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gray T. C., Ephrussi-Taylor H. Genetic recombination in DNA-induced transformation of pneumococcus. V. The sbsence of interference, and evidence for the selective elimination of certain donor sites from the final recombinants. Genetics. 1967 Sep;57(1):125–153. doi: 10.1093/genetics/57.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gurney T., Jr, Fox M. S. Physical and genetic hybrids formed in bacterial transformation. J Mol Biol. 1968 Feb 28;32(1):83–100. doi: 10.1016/0022-2836(68)90147-2. [DOI] [PubMed] [Google Scholar]
  4. Louarn J. M., Sicard A. M. Sur la sensibilité différentielle des marqueurs génétiques aux rayonnements ultraviolets, et les mécanismes de réactivation au cours de la transformation chez Diplococcus pneumoniae. C R Acad Sci Hebd Seances Acad Sci D. 1965 Jul 19;261(3):852–855. [PubMed] [Google Scholar]
  5. MARMUR J., ANDERSON W. F., MATTHEWS L., BERNS K., GAJEWSKA E., LANE D., DOTY P. The effects of ultraviolet light on the biological and physical chemical properties of deoxyribonucleic acids. J Cell Comp Physiol. 1961 Dec;58(3):33–55. doi: 10.1002/jcp.1030580406. [DOI] [PubMed] [Google Scholar]
  6. MARTIN C., EPHRUSSI-TAYLOR H. EFFECTS OF PARTIAL DEUTERATION ON TRANSFORMING DNA OF PNEUMOCOCCUS. Biochim Biophys Acta. 1964 Mar 23;80:411–421. doi: 10.1016/0926-6550(64)90143-4. [DOI] [PubMed] [Google Scholar]
  7. OTTOLENGHI E., HOTCHKISS R. D. Release of genetic transforming agent from pneumococcal cultures during growth and disintegration. J Exp Med. 1962 Oct 1;116:491–519. doi: 10.1084/jem.116.4.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Peterson J. M., Guild W. R. Fractionated strands of bacterial deoxyribonucleic acid. 3. Transformation efficiencies and rates of phenotypic expression. J Bacteriol. 1968 Dec;96(6):1991–1996. doi: 10.1128/jb.96.6.1991-1996.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RAVIN A. W. Reciprocal capsular transformations of pneumococci. J Bacteriol. 1959 Mar;77(3):296–309. doi: 10.1128/jb.77.3.296-309.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sicard A. M., Ephrussi-Taylor H. Genetic recombination in DNA-induced transformation of Pneumococcus. II. Mapping the amiA region. Genetics. 1965 Dec;52(6):1207–1227. doi: 10.1093/genetics/52.6.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sirotnak F. M., Hachtel S. L. Increased dihydrofolate reductase synthess in Diplococcus pneumoniae following translatable alteration of the structural gene. I. Genotype derivation and recombinational analyses. Genetics. 1969 Feb;61(2):293–312. doi: 10.1093/genetics/61.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES