Abstract
Salmonella phage P22 has been used in the construction of three sorts of specialized transducing phage: P22 proAB, P22 proABlac and P22 argF. The bacterial genes carried are derived from E. coli K12. Since E. coli and Salmonella chromosomes recombine very poorly, E. coli genes cannot be transduced into Salmonella recipients by P22's generalized transduction mechanism. Therefore, stable inheritance of E. coli material provides a means of detecting specialized transduction. Formation of these phages was possible because the P22 prophage recognizes an attachment site in the E. coli F' prolac episome. Salmonella strains carrying the F' prolac episome can be lysogenized by P22 so as to leave the prophage inserted into the E. coli material of the F' factor. Improper prophage excision can then lead to formation of P22 specialized phages carrying E. coli genetic material.
Full Text
The Full Text of this article is available as a PDF (1.4 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berkowitz D., Hushon J. M., Whitfield H. J., Jr, Roth J., Ames B. N. Procedure for identifying nonsense mutations. J Bacteriol. 1968 Jul;96(1):215–220. doi: 10.1128/jb.96.1.215-220.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Botstein D., Matz M. J. A recombination function essential to the growth of bacteriophage P22. J Mol Biol. 1970 Dec 28;54(3):417–440. doi: 10.1016/0022-2836(70)90119-1. [DOI] [PubMed] [Google Scholar]
- Chan R. K., Botstein D. Genetics of bacteriophage P22. I. Isolation of prophage deletions which affect immunity to superinfection. Virology. 1972 Jul;49(1):257–267. doi: 10.1016/s0042-6822(72)80027-8. [DOI] [PubMed] [Google Scholar]
- Chan R. K., Botstein D., Watanabe T., Ogata Y. Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate. Virology. 1972 Dec;50(3):883–898. doi: 10.1016/0042-6822(72)90442-4. [DOI] [PubMed] [Google Scholar]
- Chou J. Y., Martin R. G. Purine phosphoribosyltransferases of Salmonella typhimurium. J Bacteriol. 1972 Nov;112(2):1010–1013. doi: 10.1128/jb.112.2.1010-1013.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HERSHEY A. D., CHASE M. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol. 1952 May;36(1):39–56. doi: 10.1085/jgp.36.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Israel V. The production of inactive phage P22 particles following induction. Virology. 1967 Oct;33(2):317–322. doi: 10.1016/0042-6822(67)90150-x. [DOI] [PubMed] [Google Scholar]
- Itikawa H., Demerec M. Salmonella typhimurium proline mutants. J Bacteriol. 1968 Mar;95(3):1189–1190. doi: 10.1128/jb.95.3.1189-1190.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Legrain C., Halleux P., Stalon V., Glansdorff N. The dual genetic control of ornithine carbamolytransferase in Escherichia coli. A case of bacterial hybrid enzymes. Eur J Biochem. 1972 May;27(1):93–102. doi: 10.1111/j.1432-1033.1972.tb01814.x. [DOI] [PubMed] [Google Scholar]
- Rhoades M., MacHattie L. A., Thomas C. A., Jr The P22 bacteriophage DNA molecule. I. The mature form. J Mol Biol. 1968 Oct 14;37(1):21–40. doi: 10.1016/0022-2836(68)90071-5. [DOI] [PubMed] [Google Scholar]
- Smith-Keary P. F. Restricted trandsuction by bacteriophage P22 in Salmonella typhimurium. Genet Res. 1966 Aug;8(1):73–82. doi: 10.1017/s0016672300009927. [DOI] [PubMed] [Google Scholar]
- Syvanen J. M., Roth J. R. Structural genes for ornithine transcarbamylase in Salmonella typhimurium and Escherichia coli K-12. J Bacteriol. 1972 Apr;110(1):66–70. doi: 10.1128/jb.110.1.66-70.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
- Watanabe T., Ogata Y., Chan R. K., Botstein D. Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage P22. Virology. 1972 Dec;50(3):874–882. doi: 10.1016/0042-6822(72)90441-2. [DOI] [PubMed] [Google Scholar]