Abstract
Crosses have been performed which identify phage mutants (chi) which cause recombinational hot spot activity in λ. The hot spot activity is found in crosses of red- gam- chi- strains in rec+ hosts; in the crosses reported here, both the chi- mutations and the hot spot are located near the right end of the chromosome. The hot spot occurs in standard crosses as well as under conditions which block DNA synthesis, and is dependent on a functional host recB gene.—The chi mutation is shown to be dominant, but the tests do not show whether chi is a gene or a site.
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Selected References
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- Enquist L. W., Skalka A. Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec. J Mol Biol. 1973 Apr 5;75(2):185–212. doi: 10.1016/0022-2836(73)90016-8. [DOI] [PubMed] [Google Scholar]
- Fangman W. L., Novick A. Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA. Genetics. 1968 Sep;60(1):1–17. doi: 10.1093/genetics/60.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feiss M., Margulies T. On maturation of the bacteriophage lambda chromosome. Mol Gen Genet. 1973 Dec 31;127(4):285–295. doi: 10.1007/BF00267099. [DOI] [PubMed] [Google Scholar]
- Freifelder D., Chud L., Levine E. E. Requirement for maturation of Escherichia coli bacteriophage lambda. J Mol Biol. 1974 Mar 15;83(4):503–509. doi: 10.1016/0022-2836(74)90510-5. [DOI] [PubMed] [Google Scholar]
- Lindahl G., Sironi G., Bialy H., Calendar R. Bacteriophage lambda; abortive infection of bacteria lysogenic for phage P2. Proc Natl Acad Sci U S A. 1970 Jul;66(3):587–594. doi: 10.1073/pnas.66.3.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McMilin K. D., Russo V. E. Maturation and recombination of bacteriophage lambda DNA molecules in the absence of DNA duplication. J Mol Biol. 1972 Jul 14;68(1):49–55. doi: 10.1016/0022-2836(72)90261-6. [DOI] [PubMed] [Google Scholar]
- McMilin K. D., Stahl M. M., Stahl F. W. Rec-mediated recombinational hot spot activity in bacteriophage lambda. I. Hot spot activity associated with spi-deletions and bio substitutions. Genetics. 1974 Jul;77(3):409–423. doi: 10.1093/genetics/77.3.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakaki Y., Karu A. E., Linn S., Echols H. Purification and properties of the gamma-protein specified by bacteriophage lambda: an inhibitor of the host RecBC recombination enzyme. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2215–2219. doi: 10.1073/pnas.70.8.2215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stahl F. W., McMilin K. D., Stahl M. M., Malone R. E., Nozu Y., Russo V. E. A role for recombination in the production of "free-loader" lambda bacteriophage particles. J Mol Biol. 1972 Jul 14;68(1):57–67. doi: 10.1016/0022-2836(72)90262-8. [DOI] [PubMed] [Google Scholar]
- Stahl F. W., McMilin K. D., Stahl M. M., Nozu Y. An enhancing role for DNA synthesis in formation of bacteriophage lambda recombinants. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3598–3601. doi: 10.1073/pnas.69.12.3598. [DOI] [PMC free article] [PubMed] [Google Scholar]