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. 1974 Sep;78(1):81–89. doi: 10.1093/genetics/78.1.81

Misrepair Mutagenesis in Bacteriophage T4

Ronald R Green 1, John W Drake 1
PMCID: PMC1213218  PMID: 4374410

Abstract

The T4 mutations px, y and 1206 inactivate an error-prone recombination-like repair system, reducing or abolishing mutagenesis by UV irradiation, MMS, and white light irradiation in the presence of the photosensitizer 8MOP. Both px and y increase some spontaneous mutation rates and slightly enhance proflavin mutagenesis; neither mutation affects thymineless or 2AP mutagenesis appreciably, but both mildly enhance 5BU mutagenesis. The mutation hm promotes UV, MMS, photodynamic, thymineless, and base analog mutagenesis, in addition to spontaneous base pair substitution mutation. It does not, however, markedly affect proflavin mutagenesis. The px mutation maps in the vicinity of genes 41-56, and the hm mutation maps in the vicinity of genes rI-v.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyle J. M., Symonds N. Radiation-sensitive mutants of T4D. I. T4y: a new radiation-sensitive mutant; effect of the mutation on radiation survival, growth and recombination. Mutat Res. 1969 Nov-Dec;8(3):431–439. doi: 10.1016/0027-5107(69)90060-8. [DOI] [PubMed] [Google Scholar]
  2. Bridges B. A., Ashwood-Smith M. J., Munson R. J. Susceptibility of mild thermal and of ionizing radiation damage to the same recovery mechanisms in Escherichia coli. Biochem Biophys Res Commun. 1969 Apr 29;35(2):193–196. doi: 10.1016/0006-291x(69)90266-6. [DOI] [PubMed] [Google Scholar]
  3. Bridges B. A., Law J., Munson R. J. Mutagenesis in Escherichia coli. II. Evidence for a common pathway for mutagenesis by ultraviolet light, ionizing radiation and thymine deprivation. Mol Gen Genet. 1968;103(3):266–273. doi: 10.1007/BF00273698. [DOI] [PubMed] [Google Scholar]
  4. Drake J. W., McGuire J. Properties of r mutants of bacteriophage T4 photodynamically induced in the presence of thiopyronin and psoralen. J Virol. 1967 Apr;1(2):260–267. doi: 10.1128/jvi.1.2.260-267.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. George R. H. Neonatal meningitis caused by Citrobacter koseri. J Clin Pathol. 1973 Jul;26(7):552–552. doi: 10.1136/jcp.26.7.552-c. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hill R. F. Synergistic effect of an Escherichia coli mutator gene on mutagenesis by ultraviolet radiation and by alkylating agents. Mutat Res. 1972 Jan;14(1):27–31. doi: 10.1016/0027-5107(72)90104-2. [DOI] [PubMed] [Google Scholar]
  7. Hill T., Prakash L., Strauss B. Mutagen stability of alkylation-sensitive mutants of Bacillus subtilis. J Bacteriol. 1972 Apr;110(1):47–55. doi: 10.1128/jb.110.1.47-55.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kilbey B. J., de Serres F. J., Malling H. V. Identification of the genetic alteration at the molecular level of ultraviolet light-induced ad-3B mutants in Neurospora crassa. Mutat Res. 1971 May;12(1):47–56. doi: 10.1016/0027-5107(71)90071-6. [DOI] [PubMed] [Google Scholar]
  9. Meistrich M. L., Drake J. W. Mutagenic effects of thymine dimers in bacteriophage T4. J Mol Biol. 1972 Apr 28;66(1):107–114. doi: 10.1016/s0022-2836(72)80009-3. [DOI] [PubMed] [Google Scholar]
  10. Nasim A. Repair-mechanisms and radiation-induced mutations in fission yeast. Genetics. 1968 Jul;59(3):327–333. doi: 10.1093/genetics/59.3.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rupp W. D., Wilde C. E., 3rd, Reno D. L., Howard-Flanders P. Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli. J Mol Biol. 1971 Oct 14;61(1):25–44. doi: 10.1016/0022-2836(71)90204-x. [DOI] [PubMed] [Google Scholar]
  12. Siegel E. C. Ultraviolet-sensitive mutator strain of Escherichia coli K-12. J Bacteriol. 1973 Jan;113(1):145–160. doi: 10.1128/jb.113.1.145-160.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smirnov G. B., Skavronskaya A. G. Location of uvr502 mutation on the chromosome of Escherichia coli K-12. Mol Gen Genet. 1971;113(3):217–221. doi: 10.1007/BF00339541. [DOI] [PubMed] [Google Scholar]
  14. Smith M. D., Green R. R., Ripley L. S., Drake J. W. Thymineless mutagenesis in bacteriophage T4. Genetics. 1973 Jul;74(3):393–403. doi: 10.1093/genetics/74.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith S. M., Symonds N. The unexpected location of a gene conferring abnormal radiation sensitivity on phage T4. Nature. 1973 Feb 9;241(5389):395–396. doi: 10.1038/241395a0. [DOI] [PubMed] [Google Scholar]
  16. Symonds N., Heindl H., White P. Radiation sensitive mutants of phage T4. A comparative study. Mol Gen Genet. 1973 Feb 2;120(3):253–259. doi: 10.1007/BF00267156. [DOI] [PubMed] [Google Scholar]
  17. van den Ende P., Symonds N. The isolation and characterization of a T4 mutant partially defective in recombination. Mol Gen Genet. 1972;116(3):239–247. doi: 10.1007/BF00269768. [DOI] [PubMed] [Google Scholar]

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