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. 1974 Oct;78(2):645–659. doi: 10.1093/genetics/78.2.645

Regulation of Phosphate Metabolism in NEUROSPORA CRASSA: Isolation of Mutants Deficient in the Repressible Alkaline Phosphatase

Mary K Gleason 1, Robert L Metzenberg 1
PMCID: PMC1213225  PMID: 4280980

Abstract

Mutants of Neurospora crassa have been isolated that lack the repressible alkaline phosphatase, but, unlike nuc-1 and nuc-2 mutants, are able to make the repressible acid phosphatase and the repressible phosphate permease under conditions of derepression (phosphate deprivation). The new mutants, called pho-2, map in Linkage Group V, and are unlinked to the putative control mutants, nuc-1, nuc-2-pconc, and pregc. Three of the pho-2 mutants do not make detectable amounts of repressible alkaline phosphatase, but the fourth makes about 1% of the level found in wild type. The small amount of alkaline phosphatase made by this strain appears to be qualitatively similar or identical to the wild-type enzyme, as judged by electrophoretic mobility, heat stability, and titration with specific antibody to the wild-type enzyme. Several revertants of this strain have been examined in the same way, and the alkaline phosphatase of these strains also appears to be qualitatively normal. Reversion events can occur at, or near, the pho-2 locus, but also occur in at least two unlinked sites (suppressor mutations). One suppressor maps very close to nuc-1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atwood K C, Mukai F. Nuclear Distribution in Conidia of Neurospora Heterokaryons. Genetics. 1955 Jul;40(4):438–443. doi: 10.1093/genetics/40.4.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ishikawa T., Toh-E A., Uno I., Hasunuma K. Isolation and characterization of nuclease mutants in Neurospora crassa. Genetics. 1969 Sep;63(1):75–92. doi: 10.1093/genetics/63.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kadner R. J., Nyc J. F. Repressible alkaline phosphatase in Neurospora crassa. 3. Enzymatic properties. J Biol Chem. 1969 Oct 10;244(19):5125–5130. [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Lehman J. F., Gleason M. K., Ahlgren S. K., Metzenberg R. L. Regulation of phosphate metabolism in Neurospora crassa. Characterization of regulatory mutants. Genetics. 1973 Sep;75(1):61–73. doi: 10.1093/genetics/75.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Metzenberg R. L., Gleason M. K., Littlewood B. S. Genetic control of alkaline phosphatase synthesis in Neurospora: the use of partial diploids in dominance studies. Genetics. 1974 May;77(1):25–43. doi: 10.1093/genetics/77.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Metzenberg R. L. Repair of multiple defects of a regulatory mutant of Neurospora by high osmotic pressure and by reversion. Arch Biochem Biophys. 1968 May;125(2):532–541. doi: 10.1016/0003-9861(68)90611-5. [DOI] [PubMed] [Google Scholar]

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