Abstract
Two new mutant genes in Neurospora crassa prevent the formation of free macroconidia from proconidial chains. These genes, called conidial separation-1 and conidial separation-2, are phase-specific, playing no role in either the sexual life cycle or other aspects of the asexual life cycle. A cell-wall-associated autolytic activity was found to increase in wild-type cultures at the time of active formation of free conidia from proconidial chains; no such increase was detected in mutant cultures. It appears that the products of these genes are both essential for and unique to macroconidiation.
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