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. 1975 Apr;79(4):573–582. doi: 10.1093/genetics/79.4.573

Genetic Control of Radiation Sensitivity in SCHIZOSACCHAROMYCES POMBE

A Nasim 1,2, B P Smith 1,2
PMCID: PMC1213296  PMID: 166019

Abstract

Genetic analysis of a large number of radiation-sensitive mutants of S. pombe, isolated in different laboratories, showed that these isolates represent 22 non-allelic loci. The mutants were shown to fall into three distinct classes concerning response to UV and ionizing radiation, including two mutants which are primarily sensitive to ionizing radiation but not to UV. Single-gene mutants were crossed to obtain supersensitive double mutants. Such double mutants showed a marked increase in sensitivity to a variety of inactivating agents as compared to the parental strains. The isolation of three classes of radiation-sensitive mutants and the construction of double mutants implies the presence of multiple pathways in S. pombe for repair of radiation-induced damage. The bearing of these data on cellular repair mechanisms in eukaryotes is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang L. T., Tuveson R. W. Ultraviolet-sensitive mutants in Neurospora crassa. Genetics. 1967 Aug;56(4):801–810. doi: 10.1093/genetics/56.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cox B. S., Parry J. M. The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast. Mutat Res. 1968 Jul-Aug;6(1):37–55. doi: 10.1016/0027-5107(68)90101-2. [DOI] [PubMed] [Google Scholar]
  3. Davies D. R. UV-sensitive mutants of Chlamydomonas reinhardi. Mutat Res. 1967 Nov-Dec;4(6):765–770. doi: 10.1016/0027-5107(67)90085-1. [DOI] [PubMed] [Google Scholar]
  4. Fabre F. Relation between repair mechanisms and induced mitotic recombination after UV irradiation, in the yeast Schizosaccharomyces pombe. Effects of caffeine. Mol Gen Genet. 1972;117(2):153–166. doi: 10.1007/BF00267612. [DOI] [PubMed] [Google Scholar]
  5. Haefnar K., Howery L. Gene-controlled UV-sensitivity in Schizosaccharomyces pombe. Mutat Res. 1967 Mar-Apr;4(2):219–221. doi: 10.1016/0027-5107(67)90075-9. [DOI] [PubMed] [Google Scholar]
  6. Khan N. A., Brendel M., Haynes R. H. Supersensitive double mutants in yeast. Mol Gen Genet. 1970;107(4):376–378. doi: 10.1007/BF00441200. [DOI] [PubMed] [Google Scholar]
  7. Nakai S., Matsumoto S. Two types of radiation-sensitive mutant in yeast. Mutat Res. 1967 Mar-Apr;4(2):129–136. doi: 10.1016/0027-5107(67)90064-4. [DOI] [PubMed] [Google Scholar]
  8. Nasim A. Repair-mechanisms and radiation-induced mutations in fission yeast. Genetics. 1968 Jul;59(3):327–333. doi: 10.1093/genetics/59.3.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nasim A., Saunders A. S. Spontaneous frequencies of lethal-sectoring and mutation in radiation-sensitive strains of Schizosaccharomyces pombe. Mutat Res. 1968 Nov-Dec;6(3):475–478. doi: 10.1016/0027-5107(68)90066-3. [DOI] [PubMed] [Google Scholar]
  10. Nasim A., Smith B. P. Dark repair inhibitors and pathways for repair of radiation damage in Schizosaccharomyces pombe. Mol Gen Genet. 1974;132(1):13–22. doi: 10.1007/BF00268226. [DOI] [PubMed] [Google Scholar]
  11. Resnick M. A. Genetic control of radiation sensitivity in Saccharomyces cerevisiae. Genetics. 1969 Jul;62(3):519–531. doi: 10.1093/genetics/62.3.519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schroeder A. L. Ultraviolet-sensitive mutants of Neurospora. I. Genetic basis and effect on recombination. Mol Gen Genet. 1970;107(4):291–304. doi: 10.1007/BF00441192. [DOI] [PubMed] [Google Scholar]
  13. Schüpbach M. The isolation and genetic classification of UV-sensitive mutants of Schizosaccharomyces pombe. Mutat Res. 1971 Apr;11(4):361–371. [PubMed] [Google Scholar]
  14. Shahin M. M., Gentner N. E., Nasim A. The effect of liquid holding in Schizosaccharomyces pombe strains after gamma and ultraviolet irradiation. Radiat Res. 1973 Feb;53(2):216–225. [PubMed] [Google Scholar]
  15. Snow R. Mutants of yeast sensitive to ultraviolet light. J Bacteriol. 1967 Sep;94(3):571–575. doi: 10.1128/jb.94.3.571-575.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wright P. J., Pateman J. A. Ultraviolet-light sensitive mutants of Aspergillus nidulans. Mutat Res. 1970 Jun;9(6):579–587. doi: 10.1016/0027-5107(70)90103-x. [DOI] [PubMed] [Google Scholar]

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