Abstract
The effects of monosomy of specific chromosomes on the fatty acid composition of maize embryos were studied. A novel technique was developed to obtain fatty acid profiles of single embryos without reducing the viability of the sampled kernels. Monosomic 2 embryos had significantly more oleic acid and significantly less linoleic acid than diploid control embryos. Since the conversion of oleic acid to linoleic acid is a single-enzyme-mediated reaction, we suggest that a gene involved in linoleic acid biosynthesis is located on chromosome 2. Additional consistent variations were found in other monosomic types. This study demonstrates that monosomic analysis can be used to study gene dosage effects at the biochemical level.
Full Text
The Full Text of this article is available as a PDF (924.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abdelkader A. B., Cherif A., Demandre C., Mazliak P. The oleyl-coenzyme-A desaturase of potato tubers. Enzymatic properties, intracellular localization and induction during "aging" of tuber slices. Eur J Biochem. 1973 Jan 3;32(1):155–165. doi: 10.1111/j.1432-1033.1973.tb02592.x. [DOI] [PubMed] [Google Scholar]
- Baker N., Lynen F. Factors involved in fatty acyl CoA desaturation by fungal microsomes. The relative roles of acyl CoA and phospholipids as substrates. Eur J Biochem. 1971 Mar 11;19(2):200–210. doi: 10.1111/j.1432-1033.1971.tb01305.x. [DOI] [PubMed] [Google Scholar]
- Harris R. V., James A. T. Linoleic and alpha-linolenic acid biosynthesis in plant leaves and green alga. Biochim Biophys Acta. 1965 Dec 2;106(3):456–464. doi: 10.1016/0005-2760(65)90062-7. [DOI] [PubMed] [Google Scholar]
- JAMES A. T. The biosynthesis of long-chain saturated and unsaturated fatty acids in isolated plant leaves. Biochim Biophys Acta. 1963 Feb 19;70:9–19. doi: 10.1016/0006-3002(63)90714-5. [DOI] [PubMed] [Google Scholar]