Abstract
A small fraction (about 0.5%) of the transformants for a particular marker of B. subtilis (ilvA4; most probably a deletion) were found to be relatively unstable merodiploids. They possess a redundancy of the metB–ilvA chromosome segment. When their DNA is used as donor in transformation a merodiploid condition for the whole of this segment is created in all ilvA4+ transformants. For several of the duplicated loci both copies often are of recipient strain origin. Markers originally belonging to different copies of the diploidized region can be contransferred in PBS1-mediated transduction. The data are well in agreement with the hypothesis that the merodiploids carry a tandem duplication. An alternative hypothesis which does not call for integration of the exogenote within the recipient chromosome was also considered. Models are proposed for interpreting the segregation of the merodiploids, the transmission of the diploid state and its generation during transformation of the ilvA4 marker by wild-type DNA.
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- Adams A., Oishi M. Genetic properties of arsenate sensitive mutants of Bacillus subtilis 168. Mol Gen Genet. 1972;118(4):295–310. doi: 10.1007/BF00333565. [DOI] [PubMed] [Google Scholar]
- Adams A. Transformation and transduction of a large deletion mutation in Bacillus subtilis. Mol Gen Genet. 1972;118(4):311–320. doi: 10.1007/BF00333566. [DOI] [PubMed] [Google Scholar]
- BARAT M., ANAGNOSTOPOULOS C., SCHNEIDER A. M. LINKAGE RELATIONSHIPS OF GENES CONTROLLING ISOLEUCINE, VALINE, AND LEUCINE BIOSYNTHESIS IN BACILLUS SUBTILIS. J Bacteriol. 1965 Aug;90:357–369. doi: 10.1128/jb.90.2.357-369.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CURTIS S. R., 3rd A STABLE PARTIAL DIPLOID STRAIN OF ESCHERICHIA COLI. Genetics. 1964 Oct;50:679–694. doi: 10.1093/genetics/50.4.679. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curtiss R., 3rd Ultraviolet-induced genetic recombination in a partially diploid strain of Escherichia coli. Genetics. 1968 Jan;58(1):9–54. doi: 10.1093/genetics/58.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hill C. W., Foulds J., Soll L., Berg P. Instability of a missense suppressor resulting from a duplication of genetic material. J Mol Biol. 1969 Feb 14;39(3):563–581. doi: 10.1016/0022-2836(69)90146-6. [DOI] [PubMed] [Google Scholar]
- Hill C. W., Schiffer D., Berg P. Transduction of merodiploidy: induced duplication of recipient genes. J Bacteriol. 1969 Jul;99(1):274–278. doi: 10.1128/jb.99.1.274-278.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAHLER I., NEUMANN I. M., MARMUR J. Studies of genetic-units controlling arginine biosynthesis in Bacillus subtilis. Biochim Biophys Acta. 1963 May 28;72:69–79. [PubMed] [Google Scholar]
- Ravin A. W., Takahashi E. A. Merodiploid ribosomal loci arising by transformation and mutation in pneumococcus. J Bacteriol. 1970 Jan;101(1):38–52. doi: 10.1128/jb.101.1.38-52.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rutberg B., Hoch J. A. Citric acid cycle: gene-enzyme relationships in Bacillus subtilis. J Bacteriol. 1970 Nov;104(2):826–833. doi: 10.1128/jb.104.2.826-833.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trowsdale J., Anagnostopoulos C. Evidence for the Translocation of a Chromosome Sement in Bacillus subtilis Strains Carrying the trpE26 Mutation. J Bacteriol. 1975 Jun;122(3):886–898. doi: 10.1128/jb.122.3.886-898.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]