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. 1976 Jun;83(2):201–225. doi: 10.1093/genetics/83.2.201

New Map Location of ilvO in ESCHERICHIA COLI

Bruce M Cohen 1, Elizabeth W Jones 1
PMCID: PMC1213509  PMID: 17248710

Abstract

Mutations in ilvO, the operator site for operon A of the ilv region of E. coli K-12, are reported to cause a cis dominant derepression of the operon A gene products and to map between ilvC and ilvA. Studies reported here demonstrate that the F25 episome which does not carry the region between ilvC and ilvA but only ilvE, ilvD and a part of ilvA, can transfer ilvO genetic material. Also, genes of operon A on the F25 episome respond normally to a derepression signal. It is proposed that ilvO is on the F25 episome. Five-point crosses demonstrate that mutations in ilvO map nearer to ilvE than to ilvA. Considering this and other evidence, it is proposed that the gene order in the ilv region is CADEO, not COADE, with transcription and translation in operon A being from ilvE to ilvA not ilvA to ilvE. It is confirmed that mutations in ilvO cause a cis dominant derepression of the operon A gene products but it is also noted that mutations in ilvO lead to the appearance of an AHAS activity more resistant to inhibition by valine than that of the present O+ strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arfin S. M., Ratzkin B., Umbarger H. E. The metabolism of valine and isoleucine in Escherichia coli. XVII. The role of induction in the derepression of acetohydroxy acid isomeroreductase. Biochem Biophys Res Commun. 1969 Dec 4;37(6):902–908. doi: 10.1016/0006-291x(69)90216-2. [DOI] [PubMed] [Google Scholar]
  2. Botstein D., Jones E. W. Nonrandom mutagenesis of the Escherichia coli genome by nitrosoguanidine. J Bacteriol. 1969 May;98(2):847–848. doi: 10.1128/jb.98.2.847-848.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cafferata R. L., Freundlich M. Evidence for channeling of homoserine in Salmonella typhimurium. Biochim Biophys Acta. 1970 Dec 29;222(3):671–674. doi: 10.1016/0304-4165(70)90196-0. [DOI] [PubMed] [Google Scholar]
  5. Curtiss R., 3rd, Charamella L. J., Stallions D. R., Mays J. A. Parental functions during conjugation in Escherichia coli K-12. Bacteriol Rev. 1968 Dec;32(4 Pt 1):320–348. [PMC free article] [PubMed] [Google Scholar]
  6. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dwyer S. B., Umbarger H. E. Isoleucine and valine metabolism of Escherichia coli. XVI. Pattern of multivalent repression in strain K-12. J Bacteriol. 1968 May;95(5):1680–1684. doi: 10.1128/jb.95.5.1680-1684.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FREUNDLICH M., BURNS R. O., UMBARGER H. E. Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression. Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804–1808. doi: 10.1073/pnas.48.10.1804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. JACOB F., WOLLMAN E. L. Analyse des groupes de liaison génétique de différentes souches donatrices d'Escherichia coli K 12. C R Hebd Seances Acad Sci. 1957 Nov 18;245(21):1840–1843. [PubMed] [Google Scholar]
  10. LEAVITT R. I., UMBARGER H. E. Isoleucine and valine metabolism in Escherichia coli. XI. Valine inhibition of the growth of Escherichia coli strain K-12. J Bacteriol. 1962 Mar;83:624–630. doi: 10.1128/jb.83.3.624-630.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lee H. J., Otsubo E., Deonier R. C., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. V. ilv+ Deletion mutants of F14. J Mol Biol. 1974 Nov 15;89(4):585–597. doi: 10.1016/0022-2836(74)90037-0. [DOI] [PubMed] [Google Scholar]
  12. Levinthal M., Williams L. S., Umbarger H. E. Role of threonine deaminase in the regulation of isoleucine and valine biosynthesis. Nat New Biol. 1973 Nov 21;246(151):65–68. doi: 10.1038/newbio246065a0. [DOI] [PubMed] [Google Scholar]
  13. PITTARD J., LOUTIT J. S., ADELBERG E. A. GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. I. DELAY IN INITIATION OF CHROMOSOME TRANSFER. J Bacteriol. 1963 Jun;85:1394–1401. doi: 10.1128/jb.85.6.1394-1401.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sanderson K. E. Linkage map of Salmonella typhimurium, edition IV. Bacteriol Rev. 1972 Dec;36(4):558–586. doi: 10.1128/br.36.4.558-586.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wasmuth J. J., Umbarger H. E. Role for free isoleucine of glycyl-leucine in the repression of threonine deaminase in Escherichia coli. J Bacteriol. 1974 Jan;117(1):29–39. doi: 10.1128/jb.117.1.29-39.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wasmuth J., Umbarger H. E., Dempsey W. B. A role for a pyridoxne derivative in the multivalent repression of the isoleucine and valine biosynthetic enzymes. Biochem Biophys Res Commun. 1973 Mar 5;51(1):158–164. doi: 10.1016/0006-291x(73)90522-6. [DOI] [PubMed] [Google Scholar]
  17. Wechsler J. A., Adelberg E. A. Antipolarity in the ilv operon of Escherichia coli K-12. J Bacteriol. 1969 Jun;98(3):1179–1194. doi: 10.1128/jb.98.3.1179-1194.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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