Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1976 Jul;83(3):477–487.

Characterization of T4 Mutants That Partially Suppress the Inability of T4rII to Grow in Lambda Lysogens

Theodore Homyk Jr 1, Angel Rodriguez 1, Jon Weil 1
PMCID: PMC1213527  PMID: 955402

Abstract

In the course of isolating viable T4 deletions that affect plaque morphology (Homyk and Weil 1974), two closely linked point mutants, sip1 and sip2, were obtained. They map between genes t and 52, cause a reduction in plaque size and burst size, and partially suppress the lethality of rII mutants for growth in lambda lysogens. These characteristics demonstrate that sip1 and sip2 are similar to mutants previously reported by Freedman and Brenner (1972). In addition, D. Hall (personal communication) has shown that sip1 and sip2 are similar to the mutant farP85, which affects the regulation of a number of early genes ( Chace and Hall 1975).——Sip suppression of rII mutants can be demonstrated in one-step growth experiments, even when both rII genes are completely deleted. This indicates that sip mutants do not simply reduce the level of rII gene products required for growth in a lambda lysogen. Instead, they alter the growth cycle so as to partially circumvent the need for any rII products.——Mutations at two other sites, designated L1 and L2, reverse the poor phage growth caused by sip and, in the one case tested, reverse the rII-suppressing ability of sip.

Full Text

The Full Text of this article is available as a PDF (701.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benzer S. FINE STRUCTURE OF A GENETIC REGION IN BACTERIOPHAGE. Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):344–354. doi: 10.1073/pnas.41.6.344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHAMPE S. P., BENZER S. Reversal of mutant phenotypes by 5-fluorouracil: an approach to nucleotide sequences in messenger-RNA. Proc Natl Acad Sci U S A. 1962 Apr 15;48:532–546. doi: 10.1073/pnas.48.4.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chace K. V., Hall D. H. Characterization of new regulatory mutants of bacteriophage T4. II. New class of mutants. J Virol. 1975 Apr;15(4):929–945. doi: 10.1128/jvi.15.4.929-945.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doermann A. H. Lysis and Lysis Inhibition with Escherichia coli Bacteriophage. J Bacteriol. 1948 Feb;55(2):257–276. doi: 10.1128/jb.55.2.257-276.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ebisuzaki K., Campbell L. On the role of ligase in genetic recombination in bacteriophage T4. Virology. 1969 Aug;38(4):701–703. doi: 10.1016/0042-6822(69)90190-1. [DOI] [PubMed] [Google Scholar]
  7. Ennis H. L., Kievitt K. D. Association of the rIIA protein with the bacterial membrane. Proc Natl Acad Sci U S A. 1973 May;70(5):1468–1472. doi: 10.1073/pnas.70.5.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Freedman R., Brenner S. Anomalously revertible r II mutants of phage T 4 . Genet Res. 1972 Apr;19(2):165–171. doi: 10.1017/s0016672300014397. [DOI] [PubMed] [Google Scholar]
  9. GAREN A. Physiological effects of rII mutations in bacteriophage T4. Virology. 1961 Jun;14:151–163. doi: 10.1016/0042-6822(61)90190-8. [DOI] [PubMed] [Google Scholar]
  10. Homyk T., Jr, Weil J. Deletion analysis of two nonessential regions of the T4 genome. Virology. 1974 Oct;61(2):505–523. doi: 10.1016/0042-6822(74)90286-4. [DOI] [PubMed] [Google Scholar]
  11. Howard B. D. Phage lambda mutants deficient in r-II exclusion. Science. 1967 Dec 22;158(3808):1588–1589. doi: 10.1126/science.158.3808.1588. [DOI] [PubMed] [Google Scholar]
  12. Huang W. M. Membrane-associated proteins of T4-infected Escherichia coli. Virology. 1975 Aug;66(2):508–521. doi: 10.1016/0042-6822(75)90223-8. [DOI] [PubMed] [Google Scholar]
  13. Johnson J. R., Hall D. H. Characterization of new regulatory mutants of bacteriophage T4. J Virol. 1974 Mar;13(3):666–676. doi: 10.1128/jvi.13.3.666-676.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mattson T., Richardson J., Goodin D. Mutant of bacteriophage T4D affecting expression of many early genes. Nature. 1974 Jul 5;250(461):48–50. doi: 10.1038/250048a0. [DOI] [PubMed] [Google Scholar]
  15. Mukai F., Streisinger G., Miller B. The mechanism of lysis in phage T4-infected cells. Virology. 1967 Nov;33(3):398–404. doi: 10.1016/0042-6822(67)90115-8. [DOI] [PubMed] [Google Scholar]
  16. Vetter D., Sadowski P. D. Point mutants in the D2a region of bacteriophage T4 fail to induce T4 endonuclease IV. J Virol. 1974 Aug;14(2):207–213. doi: 10.1128/jvi.14.2.207-213.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weil J., Terzaghi B. The correlated occurrence of duplications and deletions in phage T4. Virology. 1970 Sep;42(1):234–237. doi: 10.1016/0042-6822(70)90259-x. [DOI] [PubMed] [Google Scholar]
  18. Weintraub S. B., Frankel F. R. Identification of the T4rIIB gene product as a membrane protein. J Mol Biol. 1972 Oct 14;70(3):589–615. doi: 10.1016/0022-2836(72)90561-x. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES