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. 1976 Jul;83(3):489–506.

Mutations Affecting Cell Division in TETRAHYMENA PYRIFORMIS . I. Selection and Genetic Analysis

Joseph Frankel 1, Leslie M Jenkins 1, F Paul Doerder 1, E Marlo Nelsen 1
PMCID: PMC1213528  PMID: 821816

Abstract

Fourteen nitrosoguanidine-induced mutations that bring about temperature-sensitive morphological abnormalities resulting from a specific effect on cell division have been isolated as heterozygous phenotypic assortants in Tetrahymena pyriformis syngen 1. Genetic analysis revealed all to be single-gene recessives. Detailed analysis of the kinetics of assortment for one of the mutated alleles revealed a rate (0.0104 pure lines per fission) consistent with that previously observed at other loci in this organism. The mutations fall into six complementation groups (mo1, mo2, mo3, mo6, mo8 , and mo12). Homozygotes of mo2 are unconditionally expressed, while all alleles of mo1, mo6, mo8, and mo12 are heat sensitive for division arrest. At the mo3 locus two alleles are heat sensitive, one is primarily cold sensitive, while two are sensitive to both heat and cold. Two out of three combinations of different mo3 alleles show conventional Mendelian segregation of conditions of expression. Different alleles of mo1, mo3, mo8, and mo12 also manifest differences in penetrance at the restrictive temperature. Despite these differences involving expression, the abnormal phenotypes themselves are locus-specific and distinctive; in the one case (mo1a and mo1 b) in which two alleles manifest somewhat different phenotypes, the F1 between them is intermediate. One additional recessive mutation (fat1) brings about a nonconditional lengthening of the cell cycle, with some arrest of cell division at the restrictive temperature. These findings demonstrate that selection of heterozygotes undergoing phenotypic assortment can be an effective method for obtaining substantial numbers of a desired class of temperature-sensitive mutations in T. pyriformis.

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Selected References

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  1. EDGAR R. S., DENHARDT G. H., EPSTEIN R. H. A COMPARATIVE GENETIC STUDY OF CONDITIONAL LETHAL MUTATIONS OF BACTERIOPHAGE T4D. Genetics. 1964 Apr;49:635–648. doi: 10.1093/genetics/49.4.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. EDGAR R. S., LIELAUSIS I. TEMPERATURE-SENSITIVE MUTANTS OF BACTERIOPHAGE T4D: THEIR ISOLATION AND GENETIC CHARACTERIZATION. Genetics. 1964 Apr;49:649–662. doi: 10.1093/genetics/49.4.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Frankel J. The effect of nucleic acid antagonists on cell division and oral organelle development in Tetrahymena pyriformis. J Exp Zool. 1965 Jun;159(1):113–147. doi: 10.1002/jez.1401590109. [DOI] [PubMed] [Google Scholar]
  4. Hartwell L. H., Culotti J., Pringle J. R., Reid B. J. Genetic control of the cell division cycle in yeast. Science. 1974 Jan 11;183(4120):46–51. doi: 10.1126/science.183.4120.46. [DOI] [PubMed] [Google Scholar]
  5. Hartwell L. H., Mortimer R. K., Culotti J., Culotti M. Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants. Genetics. 1973 Jun;74(2):267–286. doi: 10.1093/genetics/74.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaczanowski A. A single-gene-dependent abnormality of adoral membranelles in Tetrahymena pyriformis, species 1. Genetics. 1975 Dec;81(4):631–639. doi: 10.1093/genetics/81.4.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McCoy J. W. A Temperature-Sensitive Mutant in TETRAHYMENA PYRIFORMIS, Syngen 1. Genetics. 1973 May;74(1):107–114. doi: 10.1093/genetics/74.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nanney D L, Caughey P A. An Unstable Nuclear Condition in Tetrahymena Pyriformis. Genetics. 1955 May;40(3):388–398. doi: 10.1093/genetics/40.3.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nanney D L, Dubert J M. The Genetics of the H Serotype System in Variety 1 of Tetrahymena Pyriformis. Genetics. 1960 Oct;45(10):1335–1349. doi: 10.1093/genetics/45.10.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Suzuki D. T., Piternick L. K., Hayashi S., Tarasoff M., Baillie D., Erasmus U. Temperature-sensitive mutations in Drosophila melanogaster,I. Relative frequencies among gamma-ray and chemically induced sex-linked recessive lethals and semilethals. Proc Natl Acad Sci U S A. 1967 Apr;57(4):907–912. doi: 10.1073/pnas.57.4.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tasaka S. E., Suzuki D. T. Temperature-sensitive mutations in Drosophila melanogaster. XVII. Heat- and cold-sensitive lethals on chromosome 3. Genetics. 1973 Jul;74(3):509–520. doi: 10.1093/genetics/74.3.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Weindruch R. H., Doerder F. P. Age-dependent micronuclear deterioration in Tetrahymena pyriformis, syngen 1. Mech Ageing Dev. 1975 May-Aug;4(3-4):263–279. doi: 10.1016/0047-6374(75)90028-7. [DOI] [PubMed] [Google Scholar]

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