Abstract
The ability to stain mature pollen grains for the presence of alcohol dehydrogenase (ADH) activity permits the quantitation of ADH + gametophytes at frequencies below 10-6. This resolution allows reversion and genetic fine structure analyses. The rationale of pollen analysis follows Nelson's prototype studies with waxy. As with the waxy gene, revertant frequencies for seven Adh1-deficient ( Adh1-) alleles appear to be in excess of microbially derived expectations. Each of the seven Adh1- alleles were derived from one of three naturally occurring isoalleles. Based on Schwartz's protein level characterizations of the mutants' products, it was anticipated that the seven Adh1- alleles should recombine to yield ADH+ cistrons in certain pairwise combinations. This expectation was not met. The parental "wild-type" isoalleles from which the mutants were derived appear to be structurally divergent. The discussion interprets these data in view of understanding naturally occurring cistronic variation.
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Selected References
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- Sherman F., Jackson M., Liebman S. W., Schweingruber A. M., Stewart J. W. A deletion map of cyc1 mutants and its correspondence to mutationally altered iso-1-cytochromes c of yeast. Genetics. 1975 Sep;81(1):51–73. doi: 10.1093/genetics/81.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wallace B., Kass T. L. On the structure of gene control regions. Genetics. 1974 Jul;77(3):541–558. doi: 10.1093/genetics/77.3.541. [DOI] [PMC free article] [PubMed] [Google Scholar]