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. 1976 Oct;84(2):159–174. doi: 10.1093/genetics/84.2.159

The Structural Gene for α-Mannosidase-1 in DICTYOSTELIUM DISCOIDEUM

S J Free 1,2, R T Schimke 1,2, W F Loomis 1,2
PMCID: PMC1213569  PMID: 1033886

Abstract

We have isolated 4 independent mutations affecting α-mannosidase-1, a a developmentally regulated activity in Dictyostelium discoideum. Three of these result in a thermolabile α-mannosidase-1 activity. One mutation also affects the substrate affinity (Km) of the activity. In diploids these mutations show a gene dosage effect and are all alleles. The structural gene for α-mannosidase-1, as defined by these mutations, defines a new linkage group, linkage group VI. α-Mannosidase-1 is probably a homopolymer with subunits of 54,000 daltons.

We have also mapped two temperature-sensitive-for-growth mutations onto two previously defined linkage groups.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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