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. 1976 Oct;84(2):183–192. doi: 10.1093/genetics/84.2.183

Regulation of Phosphate Metabolism in NEUROSPORA CRASSA: Identification of the Structural Gene for Repressible Acid Phosphatase

Robert E Nelson 1, John F Lehman 1, Robert L Metzenberg 1
PMCID: PMC1213571  PMID: 137163

Abstract

A mutant of Neurospora crassa with an altered repressible acid phosphatase has been isolated. The enzyme is much more thermolabile than that of wild type, and has an increased Michaelis constant. Tests of allelic interactions (in partial diploids) and in vitro mixing experiments were consistent with the mutation being in the structural gene for the enzyme. This gene, pho-3, was found to be located in the right arm of Linkage Group IV (LG IV). Thus, pho-3 and the structural gene for repressible alkaline phosphatase, pho-2 (LG V), map in separate linkage groups and cannot be part of the same operon. Neither of these structural genes is linked to the known regulatory genes, nuc-1 (LG I), nuc-2 (LG II), and preg (LG II).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gleason M. K., Metzenberg R. L. Regulation of phosphate metabolism in Neurospora crassa: isolation of mutants deficient in ther repressible alkaline phosphatase. Genetics. 1974 Oct;78(2):645–659. doi: 10.1093/genetics/78.2.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hasunuma K. Repressible extracellular nucleases in Neurospora crassa. Biochim Biophys Acta. 1973 Sep 7;319(3):288–293. doi: 10.1016/0005-2787(73)90168-8. [DOI] [PubMed] [Google Scholar]
  3. Jacobs M. M., Nyc J. F., Brown D. M. Isolation and chemical properties of a repressible acid phosphatase in Neurospora crassa. J Biol Chem. 1971 Mar 10;246(5):1419–1425. [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Lowendorf H. S., Slayman C. W. Genetic regulation of phosphate transport system II in Neurospora. Biochim Biophys Acta. 1975 Nov 17;413(1):95–103. doi: 10.1016/0005-2736(75)90061-9. [DOI] [PubMed] [Google Scholar]
  6. Metzenberg R. L., Gleason M. K., Littlewood B. S. Genetic control of alkaline phosphatase synthesis in Neurospora: the use of partial diploids in dominance studies. Genetics. 1974 May;77(1):25–43. doi: 10.1093/genetics/77.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]

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