Skip to main content
Genetics logoLink to Genetics
. 1976 Oct;84(2):211–232. doi: 10.1093/genetics/84.2.211

Extension of the Limits of the Xdh Structural Element in DROSOPHILA MELANOGASTER

William Gelbart 1, Margaret McCarron 1, Arthur Chovnick 1
PMCID: PMC1213573  PMID: 826444

Abstract

Experiments expanding the array of mutants affecting the xanthine dehydrogenase (XDH) structural element in Drosophila melanogaster are described. These include rosy eye color mutants which exhibit interallelic complementation, and mutants with normal eye color but lowered levels of XDH. Evidence is presented which argues that these are structural alterations in the enzyme. Recombination experiments were performed using these mutants as well as some electrophoretic variants. The two ends of the rosy locus are marked with mutant sites which are clearly structural in nature; the XDH structural element and the rosy null mutant map are completely concordant. A possible procedure to recover control element mutants is described.

Full Text

The Full Text of this article is available as a PDF (1.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beadle G. W. A Possible Influence of the Spindle Fibre on Crossing-Over in Drosophila. Proc Natl Acad Sci U S A. 1932 Feb;18(2):160–165. doi: 10.1073/pnas.18.2.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beatriz L. W., McCarthy B. J. Messenger RNA complexity in Drosophila melanogaster. Biochemistry. 1975 Jun 3;14(11):2440–2446. doi: 10.1021/bi00682a026. [DOI] [PubMed] [Google Scholar]
  3. CHOVNICK A., SCHALET A., KERNAGHAN R. P., KRAUSS M. THE ROSY CISTRON IN DROSOPHILA MELANOGASTER: GENETIC FINE STRUCTURE ANALYSIS. Genetics. 1964 Dec;50:1245–1259. doi: 10.1093/genetics/50.6.1245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chovnick A., Ballantyne G. H., Baillie D. L., Holm D. G. Gene conversion in higher organisms: half-tetrad analysis of recombination within the rosy cistron of Drosophila melanogaster. Genetics. 1970 Oct;66(2):315–329. doi: 10.1093/genetics/66.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chovnick A., Ballantyne G. H., Holm D. G. Studies on gene conversion and its relationship to linked exchange in Drosophila melanogaster. Genetics. 1971 Oct;69(2):179–209. doi: 10.1093/genetics/69.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chovnick A. Gene conversion and transfer of genetic information within the inverted region of inversion heterozygotes. Genetics. 1973 Sep;75(1):123–131. doi: 10.1093/genetics/75.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gelbart W. M., McCarron M., Pandey J., Chovnick A. Genetic limits of the xanthine dehydrogenase structural element within the rosy locus in Drosophila melanogaster. Genetics. 1974 Nov;78(3):869–886. doi: 10.1093/genetics/78.3.869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  9. Manning J. E., Schmid C. W., Davidson N. Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome. Cell. 1975 Feb;4(2):141–155. doi: 10.1016/0092-8674(75)90121-x. [DOI] [PubMed] [Google Scholar]
  10. McCarron M., Gelbart W., Chovnick A. Intracistronic mapping of electrophoretic sites in Drosophila melanogaster: fidelity of information transfer by gene conversion. Genetics. 1974 Feb;76(2):289–299. doi: 10.1093/genetics/76.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rasch E. M., Barr H. J., Rasch R. W. The DNA content of sperm of Drosophila melanogaster. Chromosoma. 1971;33(1):1–18. doi: 10.1007/BF00326379. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES