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. 1976 Nov;84(3):507–526. doi: 10.1093/genetics/84.3.507

Characterization of Postreplication Repair in Mutagen-Sensitive Strains of DROSOPHILA MELANOGASTER

J B Boyd 1, R B Setlow 1
PMCID: PMC1213592  PMID: 826451

Abstract

Mutants of Drosophila melanogaster, with suspected repair deficiencies, were analyzed for their capacity to repair damage induced by X-rays and UV radiation. Analysis was performed on cell cultures derived from embryos of homozygous mutant stocks. Postreplication repair following UV radiation has been analyzed in mutant stocks derived from a total of ten complementation groups. Cultures were irradiated, pulse-labeled, and incubated in the dark prior to analysis by alkaline sucrose gradient centrifugation. Kinetics of the molecular weight increase in newly synthesized DNA were assayed after cells had been incubated in the presence or absence of caffeine. Two separate pathways of postreplication repair have been tentatively identified by mutants derived from four complementation groups. The proposed caffeine sensitive pathway (CAS) is defined by mutants which also disrupt meiosis. The second pathway (CIS) is caffeine insensitive and is not yet associated with meiotic functions. All mutants deficient in postreplication repair are also sensitive to nitrogen mustard. The mutants investigated display a normal capacity to repair single-strand breaks induced in DNA by X-rays, although two may possess a reduced capacity to repair damage caused by localized incorporation of high specific activity thymidine-3H. The data have been employed to construct a model for repair of UV-induced damage in Drosophila DNA. Implications of the model for DNA repair in mammals are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker B. S., Carpenter A. T. Genetic analysis of sex chromosomal meiotic mutants in Drosophilia melanogaster. Genetics. 1972 Jun;71(2):255–286. doi: 10.1093/genetics/71.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cleaver J. E., Thomas G. H. Single strand interruptions in DNA and the effects of caffeine in Chinese hamster cells irradiated with ultraviolet light. Biochem Biophys Res Commun. 1969 Jul 23;36(2):203–208. doi: 10.1016/0006-291x(69)90315-5. [DOI] [PubMed] [Google Scholar]
  3. Cox B., Game J. Repair systems in Saccharomyces. Mutat Res. 1974 Aug;26(4):257–264. doi: 10.1016/s0027-5107(74)80023-0. [DOI] [PubMed] [Google Scholar]
  4. Gelbart W. M. A new mutant controlling mitotic chromosome disjunction in Drosophila melanogaster. Genetics. 1974 Jan;76(1):51–63. doi: 10.1093/genetics/76.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lehmann A. R., Kirk-Bell S., Arlett C. F., Paterson M. C., Lohman P. H., de Weerd-Kastelein E. A., Bootsma D. Xeroderma pigmentosum cells with normal levels of excision repair have a defect in DNA synthesis after UV-irradiation. Proc Natl Acad Sci U S A. 1975 Jan;72(1):219–223. doi: 10.1073/pnas.72.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lehmann A. R., Stevens S. Postreplication repair of DNA in chick cells: studies using photoreactivation. Biochim Biophys Acta. 1975 Aug 21;402(2):179–187. doi: 10.1016/0005-2787(75)90037-4. [DOI] [PubMed] [Google Scholar]
  7. Mendelson D. The effect of caffeine on repair systems in oocytes of Drosophila melanogaster. I. Mutat Res. 1974 Feb;22(2):145–156. doi: 10.1016/0027-5107(74)90095-5. [DOI] [PubMed] [Google Scholar]
  8. Roberts J. J., Sturrock J. E., Ward K. N. The enhancement by caffeine of alkylation-induced cell death, mutations and chromosomal aberrations in Chinese hamster cells, as a result of inhibition of post-replication DNA repair. Mutat Res. 1974 Apr;26(2):129–143. doi: 10.1016/s0027-5107(74)80043-6. [DOI] [PubMed] [Google Scholar]
  9. TROSKO J. E., CHU E. H., CARRIER W. L. THE INDUCTION OF THYMINE DIMERS IN ULTRAVIOLET-IRRADIATED MAMMALIAN CELLS. Radiat Res. 1965 Apr;24:667–672. [PubMed] [Google Scholar]
  10. Wilkinson R., Kiefer J., Nias A. H. Effects of post-treatment with caffeine on the sensitivity to ultraviolet light irradiation of two lines of HeLa cells. Mutat Res. 1970 Jul;10(1):67–72. doi: 10.1016/0027-5107(70)90147-8. [DOI] [PubMed] [Google Scholar]
  11. Wolff S., Bodycote J., Painter R. B. Sister chromatid exchanges induced in Chinese hamster cells by UV irradiation of different stages of the cell cycle: the necessity for cells to pass through S. Mutat Res. 1974 Oct;25(1):73–81. doi: 10.1016/0027-5107(74)90220-6. [DOI] [PubMed] [Google Scholar]

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