Abstract
The genome of Drosophila melanogaster has been surveyed for chromosomal regions which exert a dosage effect on the activities of cAMP phosphodiesterase or cGMP phosphodiesterase. Two regions increase cAMP phosphodiesterase activity when present as duplications. A region of the X chromosome increases cAMP phosphodiesterase activity when duplicated and decreases that activity when deficient. This region has been delimited to chromomeres 3D3 and 3D4, with 3D4 being the most probable locus, and may contain a structural gene for cAMP phosphodiesterase. A region on the third chromosome, 90E–91B, increase cAMP phosphodiesterase activity when duplicated but has no affect on the activity when deficient. Two regions increase cGMP phosphodiesterase activity when present as duplications. A region of the X chromosome, 5D–9C, increases cGMP phosphodiesterase activity when duplicated, but smaller duplications covering this region fail to show such an increase, indicating that a single locus is not responsible for the increase observed for the larger duplication. A region of the third chromosome, 88C–91B, also increases cGMP phosphodiesterase activity when duplicated. Smaller duplications covering this region show smaller increases than that observed for the larger duplication, suggesting that at least three loci between 88C and 91B contribute to the observed increase by that region. Deficiencies covering region 88C–91B do not affect cGMP phosphodiesterase activity. No locus for a presumptive structural gene for cGMP phosphodiesterase has been found. Limitations of the use of segmental aneuploidy in locating structural genes for enzymes are discussed.
Full Text
The Full Text of this article is available as a PDF (818.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bourne H. R., Coffino P., Melmon K. L., Tomkins G. M., Weinstein Y. Genetic analysis of cyclic AMP in a mammalian cell. Adv Cyclic Nucleotide Res. 1975;5:771–786. [PubMed] [Google Scholar]
- D'Armiento M., Johnson G. S., Pastan I. Regulation of adenosine 3',5'-cyclic monophosphate phosphodiesterase activity in fibroblasts by intracellular concentrations of cyclic adenosine monophosphate (3T3-dibutyryl cyclic AMP-SV40-transformed cells-michaelis constants-L cells-prostaglandin E 1 ). Proc Natl Acad Sci U S A. 1972 Feb;69(2):459–462. doi: 10.1073/pnas.69.2.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EADIE G. S. On the evaluation of the constants Vm and Km in enzyme reactions. Science. 1952 Dec 19;116(3025):688–688. doi: 10.1126/science.116.3025.688. [DOI] [PubMed] [Google Scholar]
- Kiger J. A., Jr The consequences of nullosomy for a chromosomal region affecting cyclic AMP phosphodiesterase activity in Drosophila. Genetics. 1977 Apr;85(4):623–628. doi: 10.1093/genetics/85.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lindsley D. L., Sandler L., Baker B. S., Carpenter A. T., Denell R. E., Hall J. C., Jacobs P. A., Miklos G. L., Davis B. K., Gethmann R. C. Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics. 1972 May;71(1):157–184. doi: 10.1093/genetics/71.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Brien S. J., Gethmann R. C. Segmental aneuploidy as a probe for structural genes in Drosophila: mitochondrial membrane enzymes. Genetics. 1973 Sep;75(1):155–167. doi: 10.1093/genetics/75.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pledger W. J., Thompsom W. J., Strada S. J. Serum modification of cyclic nucleotide phosphodiesterase forms independent of protein synthesis. Biochem Biophys Res Commun. 1976 May 3;70(1):58–65. doi: 10.1016/0006-291x(76)91108-6. [DOI] [PubMed] [Google Scholar]
- Samir Amer M., Kreighbaum W. E. Cyclic nucleotide phosphodiesterases: properties, activators, inhibitors, structure--activity relationships, and possible role in drug development. J Pharm Sci. 1975 Jan;64(1):1–37. doi: 10.1002/jps.2600640106. [DOI] [PubMed] [Google Scholar]