Abstract
The genetic maps of the fission yeast Schizosaccharomyces pombe were extended through the use of haploidization (spontaneous or induced by m-fluorophenylalanine), as well as by tetrad, random spore and mitotic analysis. A new diploidization method utilizing a meiosis-deficient mutant and improved haploidization techniques was employed. As a result of these and previous studies, 118 genetic markers have been assigned to 3 linkage groups. Centromere markers for all 3 chromosomes were identified and genetic maps containing a total of 71 genes were constructed. Our experiments indicate that 3 is very likely to be the haploid chromosome number of S. pombe .
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- Bonatti S., Simili M., Abbondandolo A. Isolation of temperature-sensitive mutants of Schizosaccharomyces pombe. J Bacteriol. 1972 Feb;109(2):484–491. doi: 10.1128/jb.109.2.484-491.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bostock C. J. DNA synthesis in the fission yeast Schizosaccharomyces pombe. Exp Cell Res. 1970 Apr;60(1):16–26. doi: 10.1016/0014-4827(70)90484-2. [DOI] [PubMed] [Google Scholar]
- De Serres F J. Studies with Purple Adenine Mutants in Neurospora Crassa. I. Structural and Functional Complexity in the Ad-3 Region. Genetics. 1956 Sep;41(5):668–676. doi: 10.1093/genetics/41.5.668. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fischer P., Binder M., Wintersberger U. A study of the chromosomes of the yeast Schizosaccharomyces pombe by light and electron microscopy. Exp Cell Res. 1975 Nov;96(1):15–22. doi: 10.1016/s0014-4827(75)80031-0. [DOI] [PubMed] [Google Scholar]
- Nurse P., Thuriaux P., Nasmyth K. Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1976 Jul 23;146(2):167–178. doi: 10.1007/BF00268085. [DOI] [PubMed] [Google Scholar]
- Thuriaux P., Minet M., Hofer F., Leupold U. Genetic analysis of antisuppressor mutants in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1976 Dec 31;142(4):251–261. doi: 10.1007/BF00271250. [DOI] [PubMed] [Google Scholar]