Abstract
The range of specificity of the rev2–1 mutation, an allele that reduces the frequency of ochre revertants induced by UV in Saccharomyces cerevisiae (Lemontt 1971a), has been investigated by examining its influence on the reversion of eleven well-defined and contrasting cyc1 mutations. We have shown, in support of a suggestion of Lemontt (1971a), that the REV2 gene product is concerned only with the reversion of ochre alleles; it plays virtually no role in the reversion of amber, missense or frameshift mutations. We have also shown that its effect is specific and confined to only some highly revertible ochre alleles. The REV2 gene product appears to enhance reversion at these sites by facilitating the conversion of two otherwise nonmutagenic photo-products into a single premutational lesion. UV-induced killing of rev2–1 strains was found to be significantly greater on fermentable rather than on nonfermentable media.
Full Text
The Full Text of this article is available as a PDF (923.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Lawrence C. W., Christensen R. UV mutagenesis in radiation-sensitive strains of yeast. Genetics. 1976 Feb;82(2):207–232. doi: 10.1093/genetics/82.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lemontt J. F. Mutants of yeast defective in mutation induced by ultraviolet light. Genetics. 1971 May;68(1):21–33. doi: 10.1093/genetics/68.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parker J. H., Sherman F. Fine-structure mapping and mutational studies of gene controlling yeast cytochrome c1. Genetics. 1969 May;62(1):9–22. doi: 10.1093/genetics/62.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prakash L., Sherman F. Mutagenic specificity: reversion of iso-1-cytochrome c mutants of yeast. J Mol Biol. 1973 Sep 5;79(1):65–82. doi: 10.1016/0022-2836(73)90270-2. [DOI] [PubMed] [Google Scholar]
- SHERMAN F., SLONIMSKI P. P. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964 Jul 15;90:1–15. doi: 10.1016/0304-4165(64)90113-8. [DOI] [PubMed] [Google Scholar]
- Sherman F., Jackson M., Liebman S. W., Schweingruber A. M., Stewart J. W. A deletion map of cyc1 mutants and its correspondence to mutationally altered iso-1-cytochromes c of yeast. Genetics. 1975 Sep;81(1):51–73. doi: 10.1093/genetics/81.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart J. W., Sherman F. Confirmation of UAG as a nonsense codon in bakers' yeast by amino acid replacements of glutamic acid 71 in iso-1-cytochrome c. J Mol Biol. 1973 Jun 25;78(1):169–184. doi: 10.1016/0022-2836(73)90436-1. [DOI] [PubMed] [Google Scholar]