Skip to main content
NCBI home page
Genetics logoLink to Genetics
. 1978 Nov;90(3):463–474. doi: 10.1093/genetics/90.3.463

Isolation and Genetic Characterization of a Mutation Affecting Ribosomal Resistance to Cycloheximide in Tetrahymena

Manuel Ares Jr 1, Peter J Bruns 1
PMCID: PMC1213900  PMID: 730051

Abstract

A dominant mutation at a new locus affecting resistance to cycloheximide has been isolated by exploiting a synergistic relationship with a previously known mutation for cycloheximide resistance in Tetrahymena. The new mutation (ChxB) was induced in a line homozygous for ChxA and was recovered from that background by a new technique termed interrupted genomic exclusion. Segregation data from the interrupted genomic exclusion suggest that ChxA and ChxB are separate, linked loci showing 30% recombination. Minimal lethal doses of cycloheximide for the four possible combinations of the wild-type and mutant alleles of these two genes are: wild type 6 µg/ml, ChxA 125 µg/ml, ChxB 10 µg/ml, ChxA-ChxB 175 µg/ml.

Full Text

The Full Text of this article is available as a PDF (817.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen S. L. Genomic exclusion: a rapid means for inducing homozygous diploid lines in Tetrahymena pyriformis, syngen 1. Science. 1967 Feb 3;155(3762):575–577. doi: 10.1126/science.155.3762.575. [DOI] [PubMed] [Google Scholar]
  2. Bleyman L. K., Bruns P. J. Genetics of cycloheximide resistance in Tetrahymena. Genetics. 1977 Oct;87(2):275–284. doi: 10.1093/genetics/87.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bruns P. J., Brussard T. B., Kavka A. B. Isolation of homozygous mutants after induced self-fertilization in Tetrahymena. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3243–3247. doi: 10.1073/pnas.73.9.3243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bruns P. J., Sanford Y. M. Mass isolation and fertility testing of temperature-sensitive mutants in Tetrahymena. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3355–3358. doi: 10.1073/pnas.75.7.3355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Byrne B. C., Brussard T. B., Bruns P. J. Induced resistance to 6-methylpurine and cycloheximide in tetrahymena. I. Germ line mutants of T. thermophila. Genetics. 1978 Aug;89(4):695–702. doi: 10.1093/genetics/89.4.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dabbs E. R., Wittman H. G. A strain of Escherichia coli which gives rise to mutations in a large number of ribosomal proteins. Mol Gen Genet. 1976 Dec 22;149(3):303–309. doi: 10.1007/BF00268532. [DOI] [PubMed] [Google Scholar]
  7. Frankel J., Jenkins L. M., Doerder F. P., Nelsen E. M. Mutations affecting cell division in Tetrahymena pyriformis. I. Selection and genetic analysis. Genetics. 1976 Jul;83(3 PT2):489–506. [PMC free article] [PubMed] [Google Scholar]
  8. Grant P. G., Schindler D., Davies J. E. Mapping of trichodermin resistance in Saccharomyces cerevisiae: a genetic locus for a component of the 60S ribsomal subunit. Genetics. 1976 Aug;83(4):667–673. doi: 10.1093/genetics/83.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCoy J. W. A Temperature-Sensitive Mutant in TETRAHYMENA PYRIFORMIS, Syngen 1. Genetics. 1973 May;74(1):107–114. doi: 10.1093/genetics/74.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nanney D L, Dubert J M. The Genetics of the H Serotype System in Variety 1 of Tetrahymena Pyriformis. Genetics. 1960 Oct;45(10):1335–1349. doi: 10.1093/genetics/45.10.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nanney D. L., McCoy J. W. Characterization of the species of the Tetrahymena pyriformis complex. Trans Am Microsc Soc. 1976 Oct;95(4):664–682. [PubMed] [Google Scholar]
  12. Phillips R. B. Inheritance of T serotypes in Tetrahymena. Genetics. 1967 Aug;56(4):667–681. doi: 10.1093/genetics/56.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Roberts C. T., Jr, Orias E. A cycloheximide-resistant mutant of Tetrahymena pyriformis. Exp Cell Res. 1973 Oct;81(2):312–316. doi: 10.1016/0014-4827(73)90520-x. [DOI] [PubMed] [Google Scholar]
  14. Sutton C. A., Ares M., Jr, Hallberg R. L. Cycloheximide resistance can be mediated through either ribosomal subunit. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3158–3162. doi: 10.1073/pnas.75.7.3158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weindruch R. H., Doerder F. P. Age-dependent micronuclear deterioration in Tetrahymena pyriformis, syngen 1. Mech Ageing Dev. 1975 May-Aug;4(3-4):263–279. doi: 10.1016/0047-6374(75)90028-7. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES