Abstract
A gentic instability in Phycomyces is described that appears to be associated with a single nuclear gene, dar. The wild type is able to take up riboflavin and its toxic analogue, deaza-riboflavin, from nanomolar concentrations in the medium. The mutants are unable to take up riboflavin and are resistant to deaza-riboflavin. Forward and reverse mutation rates are estimated to be 4 x 10-5 and 2 x 10-3 per nuclear division. Independently arisen dar mutants do not complement in heterokaryons. The mutant alleles are almost completely recessive. The phenotype of spores is not determined cell-autonomously, but is strongly influenced by the allele ratio among the nuclei in the sporangium of origin.
Full Text
The Full Text of this article is available as a PDF (1.3 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bergman K., Burke P. V., Cerdá-Olmedo E., David C. N., Delbrück M., Foster K. W., Goodell E. W., Heisenberg M., Meissner G., Zalokar M. Phycomyces. Bacteriol Rev. 1969 Mar;33(1):99–157. doi: 10.1128/br.33.1.99-157.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cerdá-Olmedo E. Behavioral genetics of Phycomyces. Annu Rev Microbiol. 1977;31:535–547. doi: 10.1146/annurev.mi.31.100177.002535. [DOI] [PubMed] [Google Scholar]
- Perl M., Kearney E. B., Singer T. P. Transport of riboflavin into yeast cells. J Biol Chem. 1976 Jun 10;251(11):3221–3228. [PubMed] [Google Scholar]