Abstract
In this study we introduce to natural population analysis a molecular technique that involves the use of restriction endonucleases to compare mitochondrial DNA (mtDNA) sequences. We have examined the fragment patterns produced by six restriction endonucleases acting upon mtDNA isolated from 23 samples of three species of the rodent Peromyscus. Our observations confirm the following conclusions derived from previous experiments with laboratory animals: (1) mtDNA within an individual appears homogeneous; (2) at least the majority of mtDNA present in an individual is inherited from the female parent. Our experiments demonstrate for the first time that there is detectable heterogeneity in mtDNA sequences within and among natural geographic populations of a species and that this heterogeneity can readily be used to estimate relatedness between individuals and populations. Individuals collected within a single locale show less than 0.5% sequence divergence, while those collected from conspecific populations separated by 50 to 500 miles differ by approximately 1.5%. The mtDNAs of the closely related sibling species P. polionotus and P. maniculatus differ from each other by 13 to 17%; nonsibling species differ by more than 20%. Qualitative and quantitative approaches to analysis of digestion patterns are suggested. The results indicate that restriction analysis of mtDNA may become the most sensitive and powerful technique yet available for reconstructing evolutionary relationships among conspecific organisms.
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Selected References
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- Dawid I. B., Blackler A. W. Maternal and cytoplasmic inheritance of mitochondrial DNA in Xenopus. Dev Biol. 1972 Oct;29(2):152–161. doi: 10.1016/0012-1606(72)90052-8. [DOI] [PubMed] [Google Scholar]
- Fitch W. M., Margoliash E. Construction of phylogenetic trees. Science. 1967 Jan 20;155(3760):279–284. doi: 10.1126/science.155.3760.279. [DOI] [PubMed] [Google Scholar]
- Greene P. J., Heyneker H. L., Bolivar F., Rodriguez R. L., Betlach M. C., Covarrubias A. A., Backman K., Russel D. J., Tait R., Boyer H. W. A general method for the purification of restriction enzymes. Nucleic Acids Res. 1978 Jul;5(7):2373–2380. doi: 10.1093/nar/5.7.2373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jakovcic S., Casey J., Rabinowitz M. Sequence homology between mitochondrial DNAs of different eukaryotes. Biochemistry. 1975 May 20;14(10):2043–2050. doi: 10.1021/bi00681a002. [DOI] [PubMed] [Google Scholar]
- Kimura M., Ohta T. The age of a neutral mutant persisting in a finite population. Genetics. 1973 Sep;75(1):199–212. doi: 10.1093/genetics/75.1.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
- Nass M. M. The circularity of mitochondrial DNA. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1215–1222. doi: 10.1073/pnas.56.4.1215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ojala D., Attardi G. A detailed physical map of HeLa cell mitochondria DNA and its alignment with the positions of known genetic markers. Plasmid. 1977 Nov;1(1):78–105. doi: 10.1016/0147-619x(77)90010-5. [DOI] [PubMed] [Google Scholar]
- Potter S. S., Newbold J. E., Hutchison C. A., 3rd, Edgell M. H. Specific cleavage analysis of mammalian mitochondrial DNA. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4496–4500. doi: 10.1073/pnas.72.11.4496. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prager E. M., Wilson A. C. Construction of phylogenetic trees for proteins and nucleic acids: empirical evaluation of alternative matrix methods. J Mol Evol. 1978 Jun 20;11(2):129–142. doi: 10.1007/BF01733889. [DOI] [PubMed] [Google Scholar]