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. 1979 Jul;92(3):741–747. doi: 10.1093/genetics/92.3.741

ESCHERICHIA COLI K-12 Auxotrophs Induced by Insertion of the Transposable Element Tn5

Karen J Shaw 1, Claire M Berg 1
PMCID: PMC1214033  PMID: 395018

Abstract

The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess.

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Selected References

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