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. 1979 Oct;93(2):309–319.

Physiological Characterization of Polar Tn5-Induced Isoleucine-Valine Auxotrophs in ESCHERICHIA COLI K-12: Evidence for an Internal Promoter in the ilvOGEDA OPERON

Claire M Berg 1, Karen J Shaw 1, Joyce Vender 1, Maryla Borucka-Mankiewicz 1
PMCID: PMC1214080  PMID: 395023

Abstract

The properties of 22 isoleucine-valine auxotrophs induced in Escherichia coli K-12 by the transposable element, Tn5, were characterized on the basis of growth requirements, cross-feeding behavior, and enzyme activity. Mutants defective in ilvA, ilvC, ilvD and ilvE were found. Mutations in ilvE were not completely polar on ilvD and ilvA enzyme activities (that is, ilvE mutants possessed a low constitutive level of expression of the enzymes coded by ilvD and ilvA), while mutations in ilvD were completely polar on ilvA enzyme activity. The data suggest that there is an internal promoter between the sites of Tn5 insertion in ilvE and ilvD.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams C. W., Lawther R. P., Hatfield G. W. The ilvEDA operon of Escherichia coli K12 encodes only one valine-alpha-ketoglutarate transaminase activity. Biochem Biophys Res Commun. 1979 Jul 27;89(2):650–658. doi: 10.1016/0006-291x(79)90679-x. [DOI] [PubMed] [Google Scholar]
  2. Kleckner N., Chan R. K., Tye B. K., Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975 Oct 5;97(4):561–575. doi: 10.1016/s0022-2836(75)80059-3. [DOI] [PubMed] [Google Scholar]
  3. Kline E. L., Bayliss F. T. The effect of an ilvA Mu phage insertion on ilv gene expression in Escherichia coli K-12. Biochem Biophys Res Commun. 1975 Apr 21;63(4):1048–1055. doi: 10.1016/0006-291x(75)90675-0. [DOI] [PubMed] [Google Scholar]
  4. Kline E. L., Brown C. S., Coleman W. G., Jr, Umbarger H. E. Regulation of isoleucine-valine biosynthesis in an ilvDAC deletion strain of Escherichia coli K-12. Biochem Biophys Res Commun. 1974 Apr 23;57(4):1144–1151. doi: 10.1016/0006-291x(74)90816-x. [DOI] [PubMed] [Google Scholar]
  5. Kline E. L., Manross D. N., Jr, Warwick M. L. Multivalent regulation of isoleucine-valine transaminase in an Escherichia coli K-12 ilvA deletion strain. J Bacteriol. 1977 May;130(2):951–953. doi: 10.1128/jb.130.2.951-953.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  7. Lee-Peng F. C., Hermodson M. A., Kohlhaw G. B. Transaminase B from Escherichia coli: quaternary structure, amino-terminal sequence, substrate specificity, and absence of a separate valine-alpha-ketoglutarate activity. J Bacteriol. 1979 Aug;139(2):339–345. doi: 10.1128/jb.139.2.339-345.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McGilvray D., Umbarger H. E. Regulation of transaminase C synthesis in Escherichia coli: conditional leucine auxotrophy. J Bacteriol. 1974 Nov;120(2):715–723. doi: 10.1128/jb.120.2.715-723.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Shaw K. J., Berg C. M. Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5. Genetics. 1979 Jul;92(3):741–747. doi: 10.1093/genetics/92.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith J. M., Smolin D. E., Umbarger H. E. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12. Mol Gen Genet. 1976 Oct 18;148(2):111–124. doi: 10.1007/BF00268374. [DOI] [PubMed] [Google Scholar]
  11. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

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