Abstract
It is now known that partial deletions of the satellite sequences in X-chromosome heterochromatin result in a significant decrease in intrachromosomal recombination in the proximal region of the X chromosome of D. melanogaster (Yamamoto and Miklos 1978). It is important to ask then if the loss or gain of heterochromatin on the X also alters recombination in other chromosomes of the genome (interchromosomal effects). I have looked for such alterations by measuring recombination in chromosome 3. The results clearly indicate that the partial loss of X-chromosome heterochromatin not only decreases crossing over in the proximal region of the X chromosome itself, but also increases the frequency in chromosome 3, especially in the euchromatic regions around the centromere. Furthermore, the greater the deficiency of X heterochromatin, the higher is recombination in chromosome 3. This finding not only provides further evidence in support of the hypothesis that heterochromatin, in this case mainly composed of satellite DNA, regulates the recombination system, but it demonstrates that when the satellite content of one chromosome of the D. melanogaster genome is altered, there is an alteration in the crossover characteristics of other chromosomes in the same complement. If the amount of satellite DNA in a genome is being continuously altered, then one can predict that the recombination system is also being continually perturbed. Thus, the changing gene combinations produced indirectly by increases or decreases of heterochromatin are among the components available to organisms to break up or form new gene combinations upon which selection can act.
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Selected References
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- Lindsley D L, Edington C W, Von Halle E S. Sex-Linked Recessive Lethals in Drosophila Whose Expression Is Suppressed by the Y Chromosome. Genetics. 1960 Dec;45(12):1649–1670. doi: 10.1093/genetics/45.12.1649. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miklos G. L., Nankivell R. N. Telomeric satellite DNA functions in regulating recombination. Chromosoma. 1976 Jun 30;56(2):143–167. doi: 10.1007/BF00293113. [DOI] [PubMed] [Google Scholar]
- Redfield H. Egg Mortality and Interchromosomal Effects on Recombination. Genetics. 1957 Nov;42(6):712–728. doi: 10.1093/genetics/42.6.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHULTZ J., REDFIELD H. Interchromosomal effects on crossing over in Drosophila. Cold Spring Harb Symp Quant Biol. 1951;16:175–197. doi: 10.1101/sqb.1951.016.01.015. [DOI] [PubMed] [Google Scholar]
- Singh L., Purdom I. F., Jones K. W. Satellite DNA and evolution of sex chromosomes. Chromosoma. 1976 Dec 6;59(1):43–62. doi: 10.1007/BF00327708. [DOI] [PubMed] [Google Scholar]
- Steinberg A G, Fraser F C. Studies on the Effect of X Chromosome Inversions on Crossing over in the Third Chromosome of Drosophila Melanogaster. Genetics. 1944 Jan;29(1):83–103. doi: 10.1093/genetics/29.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinberg A G. The Effect of Autosomal Inversions on Crossing over in the X-Chromosome of Drosophila Melanogaster. Genetics. 1936 Sep;21(5):615–624. doi: 10.1093/genetics/21.5.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walker P. M. Origin of satellite DNA. Nature. 1971 Jan 29;229(5283):306–308. doi: 10.1038/229306a0. [DOI] [PubMed] [Google Scholar]
- Williamson J. H. Interchromosomal Effects of Autosomal Translocations on Recombination in DROSOPHILA MELANOGASTER. Genetics. 1966 Dec;54(6):1431–1440. doi: 10.1093/genetics/54.6.1431. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamoto M. Cytological studies of heterochromatin function in the Drosophila melanogaster male: autosomal meiotic paring. Chromosoma. 1979 May 10;72(3):293–328. doi: 10.1007/BF00331091. [DOI] [PubMed] [Google Scholar]
- Yamamoto M., Miklos G. L. Genetic dissection of heterochromatin in Drosophila: the role of basal X heterochromatin in meiotic sex chromosome behaviour. Chromosoma. 1977 Apr 19;60(3):283–296. doi: 10.1007/BF00329776. [DOI] [PubMed] [Google Scholar]