Abstract
Twenty-nine flagellar genes in Escherichia coli K12 have previously been assigned to three regions of the genome. Flagellar region I is located between pyrC and ptsG, region II between aroD and uvrC, and region III between uvrC and his. In this study, flagellar mutants in Escherichia coli K12 were obtained by selection for resistance to the flagellotropic phage, χ. They were analyzed in complementation tests using P1 phage-mediated transduction. In addition to the fla genes already described, eight more flagellar genes were identified. This analysis defined six more fla genes in region I (flaU, etc.), one more in region II (flbB) and one more in region III (flbC). Region I was shown to include at least 12 fla cistrons. Complementation analysis with polar Mu phage-induced Fla- mutants and with λfla phage defined four transcriptional units in region I. These were: flaU, flbA-flaW-flaV-flaK-flaX-flaL-flaY-flaM, flaZ and flaS- flaT, with transcription proceeding from left to right. The flbB gene was found to be part of an operon: flbB-flaI in region II. In region III, a previously unidentified gene flbC was located between hag and flaN.
Full Text
The Full Text of this article is available as a PDF (819.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Harayama S., Palva E. T., Hazelbauer G. L. Transposon-insertion mutants of Escherichia coli K12 defective in a component common to galactose and ribose chemotaxis. Mol Gen Genet. 1979 Mar 20;171(2):193–203. doi: 10.1007/BF00270005. [DOI] [PubMed] [Google Scholar]
- Howe M. M. Prophage deletion mapping of bacteriophage Mu-1. Virology. 1973 Jul;54(1):93–101. doi: 10.1016/0042-6822(73)90118-9. [DOI] [PubMed] [Google Scholar]
- Kleckner N., Barker D. F., Ross D. G., Botstein D. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda. Genetics. 1978 Nov;90(3):427–461. doi: 10.1093/genetics/90.3.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Komeda Y., Icho T., Iino T. Effects of galU mutation on flagellar formation in Escherichia coli. J Bacteriol. 1977 Feb;129(2):908–915. doi: 10.1128/jb.129.2.908-915.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Komeda Y., Shimada K., Iino T. Isolation of specialized lambda transducing bacteriophages for flagellar genes (fla) of Escherichia coli K-12. J Virol. 1977 Jun;22(3):654–661. doi: 10.1128/jvi.22.3.654-661.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Komeda Y., Silverman M., Simon M. Genetic analysis of Escherichia coli K-12 region I flagellar mutants. J Bacteriol. 1977 Sep;131(3):801–808. doi: 10.1128/jb.131.3.801-808.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kondoh H., Ozeki H. Deletion and amber mutants of fla loci in Escherichia coli K-12. Genetics. 1976 Nov;84(3):403–421. doi: 10.1093/genetics/84.3.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STOCKER B. A. Abortive transduction of motility in Salmonella; a nonreplicated gene transmitted through many generations to a single descendant. J Gen Microbiol. 1956 Dec;15(3):575–598. doi: 10.1099/00221287-15-3-575. [DOI] [PubMed] [Google Scholar]
- Silverman M., Matsumura P., Simon M. The identification of the mot gene product with Escherichia coli-lambda hybrids. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3126–3130. doi: 10.1073/pnas.73.9.3126. [DOI] [PMC free article] [PubMed] [Google Scholar]