Abstract
A mutant defective in the enzyme RNase P was isolated by P. Schedl and P. Primakoff (1973). The mutation rnpA49 found in this strain, which confers temperature sensitivity on carrier strains, was mapped by conjugation and transduction experiments and located around minute 82 of the E. coli map, with the suggested order rnpA bglB phoS rbsP ilv. As expected, the rnpA49 mutation is recessive. Even though this mutation is conditional, it is manifested at temperatures at which the carrier strains can grow.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Altman S. Biosynthesis of transfer RNA in Escherichia coli. Cell. 1975 Jan;4(1):21–29. doi: 10.1016/0092-8674(75)90129-4. [DOI] [PubMed] [Google Scholar]
- Apirion D. Altered ribosomes in a suppressor strain of Escherichia coli. J Mol Biol. 1966 Apr;16(2):285–301. doi: 10.1016/s0022-2836(66)80173-0. [DOI] [PubMed] [Google Scholar]
- Apirion D., Lassar A. B. A conditional lethal mutant of Escherichia coli which affects the processing of ribosomal RNA. J Biol Chem. 1978 Mar 10;253(5):1738–1742. [PubMed] [Google Scholar]
- Apirion D., Watson N. Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid. J Bacteriol. 1975 Oct;124(1):317–324. doi: 10.1128/jb.124.1.317-324.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gegenheimer P., Apirion D. Processing of rRNA by RNAase P: spacer tRNAs are linked to 16S rRNA in an RNAase P RNAase III mutant strain of E. coli. Cell. 1978 Oct;15(2):527–539. doi: 10.1016/0092-8674(78)90021-1. [DOI] [PubMed] [Google Scholar]
- Johnson S. C., Watson N., Apirion D. A lethal mutation which affects the maturation of ribosomes. Mol Gen Genet. 1976 Aug 10;147(1):29–37. doi: 10.1007/BF00337932. [DOI] [PubMed] [Google Scholar]
- Kindler P., Keil T. U., Hofschneider P. H. Isolation and characterization of a ribonuclease 3 deficient mutant of Escherichia coli. Mol Gen Genet. 1973 Oct 16;126(1):53–59. doi: 10.1007/BF00333481. [DOI] [PubMed] [Google Scholar]
- Lee S. Y., Bailey S. C., Apirion D. Small stable RNAs from Escherichia coli: evidence for the existence of new molecules and for a new ribonucleoprotein particle containing 6S RNA. J Bacteriol. 1978 Feb;133(2):1015–1023. doi: 10.1128/jb.133.2.1015-1023.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lennette E. T., Apirion D. Genetic analysis of an Escherichia coli syndrome. J Bacteriol. 1971 Dec;108(3):1322–1328. doi: 10.1128/jb.108.3.1322-1328.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pace N. R. Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes. Bacteriol Rev. 1973 Dec;37(4):562–603. doi: 10.1128/br.37.4.562-603.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schedl P., Primakoff P. Mutants of Escherichia coli thermosensitive for the synthesis of transfer RNA. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2091–2095. doi: 10.1073/pnas.70.7.2091. [DOI] [PMC free article] [PubMed] [Google Scholar]