Abstract
The flk1 mutation, which was originally isolated in the yeast Saccharomyces carlsbergenesis, causes insensitivity to catabolite repression. This mutation has been further characterized and mapped. The gene flk1 is located on chromosome III between thr4 and MAL2, 14 centimorgans from MAL2. flk1 is shown to be allelic to the pleiotropic mutants tup1, cyc9, and umr7; and flk1 is shown to exhibit an array of pleiotropic properties common to tup1, cyc9 and umr7; These results suggest that the flk1 mutation is not a specific lesion affecting catabolite repression.
Full Text
The Full Text of this article is available as a PDF (522.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Mackay V., Manney T. R. Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. II. Genetic analysis of nonmating mutants. Genetics. 1974 Feb;76(2):273–288. doi: 10.1093/genetics/76.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mahler H. R., Lin C. C. Molecular events during the release of delta-aminolevulinate dehydratase from catabolite repression. J Bacteriol. 1978 Jul;135(1):54–61. doi: 10.1128/jb.135.1.54-61.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mowshowitz D. B. Permeabilization of yeast for enzyme assays: an extremely simple method for small samples. Anal Biochem. 1976 Jan;70(1):94–99. doi: 10.1016/s0003-2697(76)80051-6. [DOI] [PubMed] [Google Scholar]
- Rothstein R. J., Sherman F. Genes affecting the expression of cytochrome c in yeast: genetic mapping and genetic interactions. Genetics. 1980 Apr;94(4):871–889. doi: 10.1093/genetics/94.4.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schamhart D. H., Ten Berge A. M., Van De Poll K. W. Isolation of a catabolite repression mutant of yeast as a revertant of a strain that is maltose negative in the respiratory-deficient state. J Bacteriol. 1975 Mar;121(3):747–752. doi: 10.1128/jb.121.3.747-752.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]