Skip to main content
Genetics logoLink to Genetics
. 1980 May;95(1):39–47. doi: 10.1093/genetics/95.1.39

A Method for Complementation Analysis of Nuclear and Chloroplast Mutants of Photosynthesis in Chlamydomonas

Pierre Bennoun 1, Arlette Masson 1, Monique Delosme 1
PMCID: PMC1214220  PMID: 17249035

Abstract

The photosynthetic properties of young zygotes of Chlamydomonas reinhardi were analyzed. In heterozygotes for two nuclear or two chloroplast mutations affecting photosynthesis, recovery of photosynthetic activity was observed that is most likely the result of intergenic complementation.——We observed that chloramphenicol inhibited the recovery of activity in double heterozygotes for mutants lacking at least one thylakoid polypeptide of chloroplast origin, while it had not effect on wild-type homozygotes. This indicates that the recovery of activity in double heterozygotes could result from the repair of existing thylakoid membranes by de novo synthesis of the missing polypeptides.

Full Text

The Full Text of this article is available as a PDF (831.9 KB).


Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES