Skip to main content
Genetics logoLink to Genetics
. 1980 May;95(1):49–61. doi: 10.1093/genetics/95.1.49

An Antisuppressor That Acts on Omnipotent Suppressors in Yeast

Susan W Liebman 1, Margaret Cavenagh 1
PMCID: PMC1214221  PMID: 7000618

Abstract

Six partially dominant antisuppressors were obtained that reduce the efficiency of two omnipotent yeast suppressors, sup45 and sup35, thought to be ribosomal ambiguity mutations. Each of these six antisuppressors was shown to fall within a single Mendelian locus, named asu9. The asu9 mutations are specific for omnipotent suppressors; they have no effect on several dominant tRNA-like suppressors. In the absence of suppressors, asu9 causes sensitivity to the aminoglycoside antibiotic, paromomycin. The properties of asu9 are consistent with the hypothesis that asu9 alters yeast ribosomal proteins.

Full Text

The Full Text of this article is available as a PDF (869.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cox B. S. A recessive lethal super-suppressor mutation in yeast and other psi phenomena. Heredity (Edinb) 1971 Apr;26(2):211–232. doi: 10.1038/hdy.1971.28. [DOI] [PubMed] [Google Scholar]
  2. Gilmore R. A., Stewart J. W., Sherman F. Amino acid replacements resulting from super-suppression of nonsense mutants of iso-1-cytochrome c from yeast. J Mol Biol. 1971 Oct 14;61(1):157–173. doi: 10.1016/0022-2836(71)90213-0. [DOI] [PubMed] [Google Scholar]
  3. McCready S. J., Cox B. S. Antisuppressors in yeast. Mol Gen Genet. 1973 Aug 28;124(4):305–320. doi: 10.1007/BF00267660. [DOI] [PubMed] [Google Scholar]
  4. Ozaki M., Mizushima S., Nomura M. Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli. Nature. 1969 Apr 26;222(5191):333–339. doi: 10.1038/222333a0. [DOI] [PubMed] [Google Scholar]
  5. Palmer E., Wilhelm J. M., Sherman F. Phenotypic suppression of nonsense mutants in yeast by aminoglycoside antibiotics. Nature. 1979 Jan 11;277(5692):148–150. doi: 10.1038/277148a0. [DOI] [PubMed] [Google Scholar]
  6. Picard-Bennoun M. Genetic evidence of ribosomal antisuppressors in Podospora anserina. Mol Gen Genet. 1976 Sep 23;147(3):299–306. doi: 10.1007/BF00582881. [DOI] [PubMed] [Google Scholar]
  7. Piper P. W., Wasserstein M., Engbaek F., Kaltoft K., Celis J. E., Zeuthen J., Liebman S., Sherman F. Nonsense suppressors of Saccharomyces cerevisiae can be generated by mutation of the tyrosine tRNA anticodon. Nature. 1976 Aug 26;262(5571):757–761. doi: 10.1038/262757a0. [DOI] [PubMed] [Google Scholar]
  8. ROMAN H., JACOB F. A comparison of spontaneous and ultraviolet-induced allelic recombination with reference to the recombination of outside markers. Cold Spring Harb Symp Quant Biol. 1958;23:155–160. doi: 10.1101/sqb.1958.023.01.019. [DOI] [PubMed] [Google Scholar]
  9. Rosset R., Gorini L. A ribosomal ambiguity mutation. J Mol Biol. 1969 Jan 14;39(1):95–112. doi: 10.1016/0022-2836(69)90336-2. [DOI] [PubMed] [Google Scholar]
  10. SHERMAN F., SLONIMSKI P. P. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964 Jul 15;90:1–15. doi: 10.1016/0304-4165(64)90113-8. [DOI] [PubMed] [Google Scholar]
  11. Sherman F., Liebman S. W., Stewart J. W., Jackson M. Tyrosine substitutions resulting from suppression of amber mutants of iso-1-cytochrome c in yeast. J Mol Biol. 1973 Jun 25;78(1):157–168. doi: 10.1016/0022-2836(73)90435-x. [DOI] [PubMed] [Google Scholar]
  12. Sherman F., Stewart J. W. Variation of mutagenic action on nonsense mutants at different sites in the iso-1-cytochrome c gene of yeast. Genetics. 1974 Sep;78(1):97–113. doi: 10.1093/genetics/78.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Singh A., Ursic D., Davies J. Phenotypic suppression and misreading Saccharomyces cerevisiae. Nature. 1979 Jan 11;277(5692):146–148. doi: 10.1038/277146a0. [DOI] [PubMed] [Google Scholar]
  14. Smirnov V. N., Kreier V. G., Lizlova L. V., Andrianova V. M., Inge-Vechtomov S. G. Recessive super-suppression in yeast. Mol Gen Genet. 1974 Mar 14;129(2):105–121. doi: 10.1007/BF00268625. [DOI] [PubMed] [Google Scholar]
  15. Smirnov V. N., Surguchov A. P., Fominykch E. S., Lizlova L. V., Saprygina T. V., Inge-Vechtomov S. G. Recessive nonsense-suppression in yeast: further characterization of a defect in translation. FEBS Lett. 1976 Jul 1;66(1):12–15. doi: 10.1016/0014-5793(76)80573-x. [DOI] [PubMed] [Google Scholar]
  16. Strigini P., Gorini L. Ribosomal mutations affecting efficiency of amber suppression. J Mol Biol. 1970 Feb 14;47(3):517–530. doi: 10.1016/0022-2836(70)90319-0. [DOI] [PubMed] [Google Scholar]
  17. Thuriaux P., Minet M., Hofer F., Leupold U. Genetic analysis of antisuppressor mutants in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1976 Dec 31;142(4):251–261. doi: 10.1007/BF00271250. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES