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. 1980 Jun;95(2):259–271. doi: 10.1093/genetics/95.2.259

Mating-Type Regulation of Methyl Methanesulfonate Sensitivity in SACCHAROMYCES CEREVISIAE

George P Livi 1, Vivian L Mackay 1
PMCID: PMC1214225  PMID: 17249036

Abstract

Heterozygosity at the mating-type locus (MAT) in Saccharomyces cerevisiae has been shown previously to enhance X-ray survival in diploid cells. We now show that a/α diploids are also more resistant to the radiomimetic agent methyl methanesulfonate (MMS) than are diploids that are homozygous at MAT (i.e., either a/a or α/α). Log-phase a/α cultures exhibit biphasic MMS survival curves, in which the more resistant fraction consists of budded cells (those cells in the S and G2 phases of the cell cycle). Survival curves for log-phase cultures of a/a or α/α diploids have little if any biphasic nature, suggesting that the enhanced S- and G2-phase repair capacity of a/α cells may be associated with heterozygosity at MAT. The survival of cells arrested at the beginning of the S phase with hydroxyurea indicates that MAT-dependent MMS repair is limited to S and G2, whereas MAT-independent repair can occur in G1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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