Abstract
The am locus of Neurospora codes for NADP-dependent glutamate dehydrogenase (GDH). Four new am mutants that produced mutationally altered GDH have been characterized. Mutant am119 is a CRM-negative, complementing mutant that maps between am2 and am1. The other three mutants are CRM formers that produce varieties of GDH that can be activated by glutamate or succinate. The GDH of am130 and am131 is similar in terms of activation properties to that of am3. The GDH of am122 requires very high concentrations of dicarboxylate for activity. The mutation in am130 maps between am14 and am2 and resulted in a replacement at residue 75 of the GDH (pro → ser). The mutation in am122 maps near am11 and apparently resulted in the replacement of the tryptophan residue at position 389 with an unknown amino acid. The mutation in am131 maps between am2 and am1.
Full Text
The Full Text of this article is available as a PDF (745.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brett M., Chambers G. K., Holder A. A., Fincham J. R., Wootton J. C. Mutational amino acid replacements in Neurospora crassa NADP-specific glutamate dehydrogenase. J Mol Biol. 1976 Sep 5;106(1):1–22. doi: 10.1016/0022-2836(76)90297-7. [DOI] [PubMed] [Google Scholar]
- Coddington A., Fincham J. R., Sundaram T. K. Multiple active varieties of Neurospora glutamate dehydrogenase formed by hybridization between two inactive mutant proteins in vivo and in vitro. J Mol Biol. 1966 Jun;17(2):503–512. doi: 10.1016/s0022-2836(66)80160-2. [DOI] [PubMed] [Google Scholar]
- ROBERTS D. B., PATEMAN J. A. IMMUNOLOGICAL STUDIES OF AMINATION DEFICIENT STRAINS OF NEUROSPORA CRASSIA. J Gen Microbiol. 1964 Feb;34:295–305. doi: 10.1099/00221287-34-2-295. [DOI] [PubMed] [Google Scholar]
- Seale T. W., Brett M., Baron A. J., Fincham J. R. Amino acid replacements resulting from suppression and missense reversion of a chain-terminator mutation in Neurospora. Genetics. 1977 Jun;86(2 Pt 1):261–274. [PMC free article] [PubMed] [Google Scholar]
- Wootton J. C., Taylor J. G., Jackson A. A., Chambers G. K., Fincham J. R. The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. The tryptic peptides. Biochem J. 1975 Sep;149(3):739–748. doi: 10.1042/bj1490739. [DOI] [PMC free article] [PubMed] [Google Scholar]