Abstract
Ninety-six isochromosomal lines of Drosophila melanogaster from a natural population were screened electrophoretically for unusual mobility variants at the alcohol dehydrogenase locus, using a total of eight conditions of acrylamide electrophoresis. No additional mobility variation was found among the 50 "slow" and 46 "fast" mobility lines beyond that detected by standard methods of electrophoresis. However, two thermostability variants recovered by R. Milkman from a natural population, whose electrophoretic mobilities were previously thought to be indistinguishable from those of "standard" alleles, are distinguishable from the standard electromorphs by these procedures. These results suggest that the Adh locus, although polymorphic, does not harbor substantial amounts of "hidden" allelic variability. This study also reports the appearance of substantial mobility variation among isogenic lines that can be induced under specific conditions of sample preparation involving the pretreatment of samples with NAD and acetone. However, genetic analysis demonstrates that this variability cannot be attributed to allelic differences at the structural locus, but instead appears to be dependent upon the concentration of the enzyme in a sample. These results are discussed in relation to the distribution of allelic variation at other enzyme loci.
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Selected References
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- Ayala F. J., Powell J. R., Tracey M. L., Mourão C. A., Pérez-Salas S. Enzyme variability in the Drosophila willistoni group. IV. Genic variation in natural populations of Drosophila willistoni. Genetics. 1972 Jan;70(1):113–139. doi: 10.1093/genetics/70.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cobbs G. Polymorphism for dimerizing ability at the esterase-5 locus in Drosophila pseudoobscura. Genetics. 1976 Jan;82(1):53–62. doi: 10.1093/genetics/82.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coyne J. A., Felton A. A. Genic Heterogeneity at Two Alcohol Dehydrogenase Loci in DROSOPHILA PSEUDOOBSCURA and DROSOPHILA PERSIMILIS. Genetics. 1977 Oct;87(2):285–304. doi: 10.1093/genetics/87.2.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coyne J. A., Felton A. A., Lewontin R. C. Extent of genetic variation at a highly polymorphic esterase locus in Drosophila pseudoobscura. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5090–5093. doi: 10.1073/pnas.75.10.5090. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coyne J. A. Lack of genic similarity between two sibling species of drosophila as revealed by varied techniques. Genetics. 1976 Nov;84(3):593–607. doi: 10.1093/genetics/84.3.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
- Jacobson K. B., Murphy J. B., Knopp J. A., Ortiz J. R. Multiple forms of drosophila alcohol dehydrogenase. 3. Conversion of one form to another by nicotinamide adenine dinucleotide or acetone. Arch Biochem Biophys. 1972 Mar;149(1):22–35. doi: 10.1016/0003-9861(72)90295-0. [DOI] [PubMed] [Google Scholar]
- Johnson G. B. Structural flexibility of isozyme variants: genetic variants in Drosophila disguised by cofactor and subunit binding. Proc Natl Acad Sci U S A. 1978 Jan;75(1):395–399. doi: 10.1073/pnas.75.1.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Langley C. H., Ito K., Voelker R. A. Linkage disequilibrium in natural populations of Drosophila melanogaster Seasonal variation. Genetics. 1977 Jun;86(2 Pt 1):447–454. [PMC free article] [PubMed] [Google Scholar]
- Langley C. H., Tobari Y. N., Kojima K. I. Linkage disequilibrium in natural populations of Drosophila melanogaster. Genetics. 1974 Nov;78(3):921–936. doi: 10.1093/genetics/78.3.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maroni G. Genetic control of alcohol dehydrogenase levels in Drosophila. Biochem Genet. 1978 Jun;16(5-6):509–523. doi: 10.1007/BF00484215. [DOI] [PubMed] [Google Scholar]
- McDonald J. F., Ayala F. J. Genetic and biochemical basis of enzyme activity variation in natural populations. I. Alcohol dehydrogenase in Drosophila melanogaster. Genetics. 1978 Jun;89(2):371–388. doi: 10.1093/genetics/89.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Milkman R. Further evidence of thermostability variation within electrophoretic mobility classes of enzymes. Biochem Genet. 1976 Apr;14(3-4):383–387. doi: 10.1007/BF00484776. [DOI] [PubMed] [Google Scholar]
- ORNSTEIN L. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964 Dec 28;121:321–349. doi: 10.1111/j.1749-6632.1964.tb14207.x. [DOI] [PubMed] [Google Scholar]
- Pipkin S. B., Franklin-Springer E., Law S., Lubega S. New studies of the alcohol dehydrogenase cline in D. melanogaster from Mexico. J Hered. 1976 Sep-Oct;67(5):258–266. doi: 10.1093/oxfordjournals.jhered.a108728. [DOI] [PubMed] [Google Scholar]
- Ramshaw J. A., Coyne J. A., Lewontin R. C. The sensitivity of gel electrophoresis as a detector of genetic variation. Genetics. 1979 Dec;93(4):1019–1037. doi: 10.1093/genetics/93.4.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sampsell B. Isolation and genetic characterization of alcohol dehydrogenase thermostability variants occurring in natural populations of Drosophila melanogaster. Biochem Genet. 1977 Oct;15(9-10):971–988. doi: 10.1007/BF00483992. [DOI] [PubMed] [Google Scholar]
- Singh R. S., Lewontin R. C., Felton A. A. Genetic heterogeneity within electrophoretic "alleles" of xanthine dehydrogenase in Drosophila pseudoobscura. Genetics. 1976 Nov;84(3):609–629. doi: 10.1093/genetics/84.3.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thörig E. W., Schoone A. A., Scharloo W. Variation between electrophoretically identical alleles at the alcohol dehydrogenase locus in Drosophila melanogaster. Biochem Genet. 1975 Oct;13(9-10):721–731. doi: 10.1007/BF00484929. [DOI] [PubMed] [Google Scholar]
- Voelker R. A., Langley C. H., Brown A. J., Ohnishi S., Dickson B., Montgomery E., Smith S. C. Enzyme null alleles in natural populations of Drosophila melanogaster: Frequencies in a North Carolina population. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1091–1095. doi: 10.1073/pnas.77.2.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]