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. 1980 Jul;95(3):611–630. doi: 10.1093/genetics/95.3.611

Ultraviolet Mutagenesis Studies of [psi], a Cytoplasmic Determinant of SACCHAROMYCES CEREVISIAE

M F Tuite 1, B S Cox 1
PMCID: PMC1214250  PMID: 7002721

Abstract

UV mutagenesis was used to probe the molecular nature of [psi], a non-mitochondrial cytoplasmic determinant of Saccharomyces cerevisiae involved in the control of nonsense suppression. The UV-induced mutation from [psi+] to [psi-] showed characteristics of forward nuclear gene mutation in terms of frequency, induction kinetics, occurrence of whole and sectored mutant clones and the effect of the stage in the growth cycle on mutation frequency. The involvement of pyrimidine dimers in the premutational lesion giving the [psi-] mutation was demonstrated by photoreactivation. UV-induced damage to the [psi] genetic determinant was shown to be repaired by nuclear-coded repair enzymes that are responsible for the repair of nuclear DNA damage. UV-induced damage to mitochondrial DNA appeared to be, at least partly, under the control of different repair processes. The evidence obtained suggests that the [psi] determinant is DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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