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. 1980 Aug;95(4):855–875. doi: 10.1093/genetics/95.4.855

Frameshift Suppression in SACCHAROMYCES CEREVISIAE. III. Isolation and Genetic Properties of Group III Suppressors

Claudia M Cummins 1,2, Richard F Gaber 1,2, Michael R Culbertson 1,2, Richard Mann 1,2, Gerald R Fink 1,2
PMCID: PMC1214273  PMID: 7009319

Abstract

Suppressors of ICR-induced mutations that exhibit behavior similar to bacterial frameshift suppressors have been identified in the yeast Saccharomyces cerevisiae. The yeast suppressors have been divided into two groups. Previous evidence indicated that suppressors of one group (Group II: SUF1, SUF3, SUF4, SUF5 and SUF6) represent mutations in the structural genes for glycyl-tRNA's. Suppressors of the other group (Group III: SUF2 and SUF7) were less well characterized. Although they suppressed some ICR-revertible mutations, they failed to suppress Group II frameshift mutations. This communication provides a more thorough characterization of the Group III suppressors and describes the isolation and properties of four new suppressors in that group (SUF8, SUF9, SUF10 and suf11).——In our original study, Group III suppressors were isolated as revertants of the Group III mutations his4–712 and his4–713. All suppressors obtained as ICR-induced revertants of these mutations mapped at the SUF2 locus near the centromere of chromosome III. Suppressors mapping at other loci were obtained in this study by analyzing spontaneous and UV-induced revertants of the Group III mutations. SUF2 and SUF10 suppress both Group III his4 mutations, whereas SUF7, SUF8, SUF9 and suf11 suppress his4–713, but not his4–712. All of the suppressors except suf11 are dominant in diploids homozygous for his4-713. The suppressors fail to suppress representative UAA, UAG and UGA nonsense mutations.——SUF9 is linked to the centromere of chromosome VI, and SUF10 is linked to the centromere of chromosome XIV. A triploid mapping procedure was used to determine the chromosome locations of SUF7 and SUF8. Subsequent standard crosses revealed linkage of SUF7 to cdc5 on chromosome XIII and linkage of SUF8 to cdc12 and pet3 on chromosome VIII.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brusick D. J. The mutagenic acitivity of ICR-170 in Saccharomyces cerevisiae. Mutat Res. 1970 Jul;10(1):11–19. doi: 10.1016/0027-5107(70)90141-7. [DOI] [PubMed] [Google Scholar]
  2. CARLSON E. A., OSTER I. I. Comparative mutagenesis of the dumpy locus in Drosophila melanogaster. II. Mutational mosaicism induced without apparent breakage by a monofunctional alkylating agent. Genetics. 1962 May;47:561–576. doi: 10.1093/genetics/47.5.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Culbertson M. R., Charnas L., Johnson M. T., Fink G. R. Frameshifts and frameshift suppressors in Saccharomyces cerevisiae. Genetics. 1977 Aug;86(4):745–764. doi: 10.1093/genetics/86.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Culbertson M. R., Underbrink K. M., Fink G. R. Frameshift suppression Saccharomyces cerevisiae. II. Genetic properties of group II suppressors. Genetics. 1980 Aug;95(4):833–853. doi: 10.1093/genetics/95.4.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fink G. R. A cluster of genes controlling three enzymes in histidine biosynthesis in Saccharomyces cerevisiae. Genetics. 1966 Mar;53(3):445–459. doi: 10.1093/genetics/53.3.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fink G. R., Styles C. A. Gene conversion of deletions in the his4 region of yeast. Genetics. 1974 Jun;77(2):231–244. doi: 10.1093/genetics/77.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hawthorne D. C. Identification of nonsense codons in yeast. J Mol Biol. 1969 Jul 14;43(1):71–75. doi: 10.1016/0022-2836(69)90079-5. [DOI] [PubMed] [Google Scholar]
  8. Liebman S. W., Sherman F. Extrachromosomal psi+ determinant suppresses nonsense mutations in yeast. J Bacteriol. 1979 Sep;139(3):1068–1071. doi: 10.1128/jb.139.3.1068-1071.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Liebman S. W., Stewart J. W., Sherman F. Serine substitutions caused by an ochre suppressor in yeast. J Mol Biol. 1975 Jun 5;94(4):595–610. doi: 10.1016/0022-2836(75)90324-1. [DOI] [PubMed] [Google Scholar]
  10. Malling H. V. The mutagenicity of the acridine mustard (ICR-170) and the structurally related compounds in Neurospora. Mutat Res. 1967 May-Jun;4(3):265–274. doi: 10.1016/0027-5107(67)90021-8. [DOI] [PubMed] [Google Scholar]
  11. Mortimer R. K., Hawthorne D. C. Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes. Genetics. 1973 May;74(1):33–54. doi: 10.1093/genetics/74.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Riddle D. L., Carbon J. Frameshift suppression: a nucleotide addition in the anticodon of a glycine transfer RNA. Nat New Biol. 1973 Apr 25;242(121):230–234. doi: 10.1038/newbio242230a0. [DOI] [PubMed] [Google Scholar]
  13. Riddle D. L., Roth J. R. Suppressors of frameshift mutations in Salmonella typhimurium. J Mol Biol. 1970 Nov 28;54(1):131–144. doi: 10.1016/0022-2836(70)90451-1. [DOI] [PubMed] [Google Scholar]
  14. Streisinger G., Okada Y., Emrich J., Newton J., Tsugita A., Terzaghi E., Inouye M. Frameshift mutations and the genetic code. This paper is dedicated to Professor Theodosius Dobzhansky on the occasion of his 66th birthday. Cold Spring Harb Symp Quant Biol. 1966;31:77–84. doi: 10.1101/sqb.1966.031.01.014. [DOI] [PubMed] [Google Scholar]
  15. Wickner R. B. Mapping chromosomal genes of Saccharomyces cerevisiae using an improved genetic mapping method. Genetics. 1979 Jul;92(3):803–821. doi: 10.1093/genetics/92.3.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yourno J., Heath S. Nature of the hisD3018 frameshift mutation in Salmonella typhimurium. J Bacteriol. 1969 Oct;100(1):460–468. doi: 10.1128/jb.100.1.460-468.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yourno J. Similarity of cross-suppressible frameshifts in Salmonella typhimurium. J Mol Biol. 1971 Nov 28;62(1):223–231. doi: 10.1016/0022-2836(71)90141-0. [DOI] [PubMed] [Google Scholar]

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