Abstract
Chi, an element that stimulates recombination via the E. coli RecBC pathway, can arise by spontaneous mutation in the transposon Tn5. When in phage λ in one orientation, the mutant transposon confers Chi+ phenotype (large plaque and a high rate of exchange near the transposon). In the other orientation, however, the transposon does not confer Chi+ phenotype. The mobility of the transposon allows us to show that the Chi+ orientation of the mutant Tn5 is the same at different locations in λ. These include a site near gene J, one in gam at 69, one to the right of gam at 73 and several to the right of R between 95.7 and 99.5. To the right of R, the mutant transposon could be found in only one orientation, that which confers Chi+ phenotype. We speculate that the other orientation of Tn5 in that locale is lethal to λ. The orientation-dependence of Chi+ phenotype also revealed that Tn5 flip-flops in λ.
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Selected References
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- Adhya S., Cleary P., Campbell A. A deletion analysis of prophage lambda and adjacent genetic regions. Proc Natl Acad Sci U S A. 1968 Nov;61(3):956–962. doi: 10.1073/pnas.61.3.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berg D. E., Davies J., Allet B., Rochaix J. D. Transposition of R factor genes to bacteriophage lambda. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628–3632. doi: 10.1073/pnas.72.9.3628. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berg D. E., Weiss A., Crossland L. Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol. 1980 May;142(2):439–446. doi: 10.1128/jb.142.2.439-446.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chattoraj D. K., Inman R. B. Tandem duplication in bacteriophage P2: electron microscopic mapping. Proc Natl Acad Sci U S A. 1974 Feb;71(2):311–314. doi: 10.1073/pnas.71.2.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAISER A. D., JACOB F. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology. 1957 Dec;4(3):509–521. doi: 10.1016/0042-6822(57)90083-1. [DOI] [PubMed] [Google Scholar]
- MESELSON M. ON THE MECHANISM OF GENETIC RECOMBINATION BETWEEN DNA MOLECULES. J Mol Biol. 1964 Sep;9:734–745. doi: 10.1016/s0022-2836(64)80178-9. [DOI] [PubMed] [Google Scholar]
- Moise H., Hosoda J. T4 gene 32 protein model for control of activity at replication fork. Nature. 1976 Feb 12;259(5543):455–458. doi: 10.1038/259455a0. [DOI] [PubMed] [Google Scholar]
- Signer E. R., Weil J. Recombination in bacteriophage lambda. I. Mutants deficient in general recombination. J Mol Biol. 1968 Jul 14;34(2):261–271. doi: 10.1016/0022-2836(68)90251-9. [DOI] [PubMed] [Google Scholar]
- Smith G. R. Deletion mutations of the immunity region of coliphage lambda. Virology. 1975 Apr;64(2):544–552. doi: 10.1016/0042-6822(75)90131-2. [DOI] [PubMed] [Google Scholar]
- Smith G. R., Schultz D. W., Crasemann J. M. Generalized recombination: nucleotide sequence homology between Chi recombinational hotspots. Cell. 1980 Mar;19(3):785–793. doi: 10.1016/s0092-8674(80)80054-7. [DOI] [PubMed] [Google Scholar]
- Sprague K. U., Faulds D. H., Smith G. R. A single base-pair change creates a Chi recombinational hotspot in bacteriophage lambda. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6182–6186. doi: 10.1073/pnas.75.12.6182. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stahl F. W., Crasemann J. M., Stahl M. M. Rec-mediated recombinational hot spot activity in bacteriophage lambda. III. Chi mutations are site-mutations stimulating rec-mediated recombination. J Mol Biol. 1975 May 15;94(2):203–212. doi: 10.1016/0022-2836(75)90078-9. [DOI] [PubMed] [Google Scholar]
- Stahl F. W., McMilin K. D., Stahl M. M., Malone R. E., Nozu Y., Russo V. E. A role for recombination in the production of "free-loader" lambda bacteriophage particles. J Mol Biol. 1972 Jul 14;68(1):57–67. doi: 10.1016/0022-2836(72)90262-8. [DOI] [PubMed] [Google Scholar]