Skip to main content
NCBI home page
Genetics logoLink to Genetics
. 1980 Oct;96(2):321–329. doi: 10.1093/genetics/96.2.321

Correlation between Map Position and Phenotype of CTI Mutants in the C Cistron of RHIZOBIUM MELILOTI Phage 16–3

Brigitta Dudás 1, László Orosz 1
PMCID: PMC1214302  PMID: 7262538

Abstract

Nine temperature-sensitive clear mutations (Cti) in the C cistron (coding for the repressor protein) of Rhizobium meliloti temperate phage 16–3 were characterized according to the inductive temperature, the immunity of cells lysogenic for these mutant phages to superinfection by homoimmune weak virulent mutants, the phenotype of double-ti mutants and interallelic complementation. The results indicate that mutations of similar phenotypic expression are clustered on the genetic map. Furthermore, it seems probable that the C cistron of the original phage 16–3 is identical to that of the independently isolated phage strain 36.

Full Text

The Full Text of this article is available as a PDF (647.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Case M. E., Giles N. H. Genetic evidence on the organization and action of the qa-1 gene product: a protein regulating the induction of three enzymes in quinate catabolism in Neurospora crassa. Proc Natl Acad Sci U S A. 1975 Feb;72(2):553–557. doi: 10.1073/pnas.72.2.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dallmann G., Orosz L., Sain B. Restriction mapping of DNA of temperate Rhizobium meliloti phage 16-3: comparison of genetic and physical maps indicates a long, genetically silent chromosomal arm. Mol Gen Genet. 1979 Nov;176(3):439–448. doi: 10.1007/BF00333109. [DOI] [PubMed] [Google Scholar]
  3. LEVINE M. Mutations in the temperate phage P22 and lysogeny in Salmonella. Virology. 1957 Feb;3(1):22–41. doi: 10.1016/0042-6822(57)90021-1. [DOI] [PubMed] [Google Scholar]
  4. Matsubara K., Kaiser A. D. Lambda dv: an autonomously replicating DNA fragment. Cold Spring Harb Symp Quant Biol. 1968;33:769–775. doi: 10.1101/sqb.1968.033.01.088. [DOI] [PubMed] [Google Scholar]
  5. Orosz L. Methods for Analysis of the C Cistron of Temperate Phage 16-3 of RHIZOBIUM MELILOTI. Genetics. 1980 Feb;94(2):265–276. doi: 10.1093/genetics/94.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Orosz L., Páy A., Dallmann G. Heterozygosis of phage 16-3 of Rhizobium meliloti: moderate level of mismatch repair or gene conversion. Mol Gen Genet. 1980;179(1):163–167. doi: 10.1007/BF00268459. [DOI] [PubMed] [Google Scholar]
  7. Orosz L., Rostás K., Hotchkiss R. D. A comparison of two-point, three-point and deletion mapping in the C cistron of rhizobiophage 16-3, with an explanation for the recombination pattern. Genetics. 1980 Feb;94(2):249–263. doi: 10.1093/genetics/94.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Orosz L., Sik T. Genetic mapping of rhizobiophage 16-3. Acta Microbiol Acad Sci Hung. 1970;17(2):185–194. [PubMed] [Google Scholar]
  9. Orosz L., Sváb Z., Kondorosi A., Sik T. Genetic studies on rhizobiophage 16-3. I. Genes and functions on the chromosome. Mol Gen Genet. 1973 Sep 27;125(4):341–350. [PubMed] [Google Scholar]
  10. Pfahl M., Stockter C., Gronenborn B. Genetic analysis of the active sites of lac repressor. Genetics. 1974 Apr;76(4):669–679. doi: 10.1093/genetics/76.4.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Platt T., Weber K., Ganem D., Miller J. H. Translational restarts: AUG reinitiation of a lac repressor fragment. Proc Natl Acad Sci U S A. 1972 Apr;69(4):897–901. doi: 10.1073/pnas.69.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ptashne M. ISOLATION OF THE lambda PHAGE REPRESSOR. Proc Natl Acad Sci U S A. 1967 Feb;57(2):306–313. doi: 10.1073/pnas.57.2.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sauer R. T., Pabo C. O., Meyer B. J., Ptashne M., Backman K. C. Regulatory functions of the lambda repressor reside in the amino-terminal domain. Nature. 1979 May 31;279(5712):396–400. doi: 10.1038/279396a0. [DOI] [PubMed] [Google Scholar]
  14. Sik T., Horváth J., Chatterjee S. Generalized transduction in Rhizobium meliloti. Mol Gen Genet. 1980;178(3):511–516. doi: 10.1007/BF00337855. [DOI] [PubMed] [Google Scholar]
  15. Valone J. A., Jr, Case M. E., Giles N. H. Constitutive mutants in a regulatory gene exerting positive control of quinic acid catabolism in Neurospora crassa. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1555–1559. doi: 10.1073/pnas.68.7.1555. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES