Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1965 May;95(2):378–386. doi: 10.1042/bj0950378

Calcium ion accumulation and volume changes of isolated liver mitochondria. Calcium ion-induced swelling

J B Chappell 1,*, A R Crofts 1,*
PMCID: PMC1214334  PMID: 14340088

Abstract

1. Liver mitochondria suspended in an iso-osmotic buffered potassium chloride medium containing an oxidizable substrate and phosphate accumulated added Ca2+. During this process H+ appeared in the medium and the mitochondrial suspension showed increased light-scattering. Respiration was markedly stimulated. 2. The addition of excess of Ca2+, respiratory inhibitors or uncoupling agents caused extensive mitochondrial swelling associated with release of Ca2+ into the suspending medium. When the suspension became anaerobic extensive swelling also occurred. Only under conditions when the addition of uncoupling agents would have produced high rates of electron transport, e.g. in the presence of succinate, was the structural integrity of the mitochondrion maintained after Ca2+ accumulation. 3. Conditions that prevented respiration-dependent Ca2+ accumulation also prevented Ca2+-induced swelling. Bovine plasma albumin was without effect, indicating that U-factor was not involved. Oligomycin together with ADP or ATP partially stabilized the mitochondria against Ca2+-induced swelling. 4. It is suggested that a `high-energy' intermediate generated by coupled electron transport is required to prevent the mitochondrial swelling that results as a consequence of Ca2+ accumulation.

Full text

PDF
378

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRIERLEY G. P., BACHMANN E., GREEN D. E. Active transport of inorganic phosphate and magnesium ions by beef heart mitochondria. Proc Natl Acad Sci U S A. 1962 Nov 15;48:1928–1935. doi: 10.1073/pnas.48.11.1928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRIERLEY G., MURER E., BACHMANN E., GREEN D. E. STUDIES ON ION TRANSPORT. II. THE ACCUMULATION OF INORGANIC PHOSPHATE AND MAGNESIUM IONS BY HEART MITOCHONDRIA. J Biol Chem. 1963 Oct;238:3482–3489. [PubMed] [Google Scholar]
  3. CHAPPELL J. B., CROFTS A. R. GRAMICIDIN AND ION TRANSPORT IN ISOLATED LIVER MITOCHONDRIA. Biochem J. 1965 May;95:393–402. doi: 10.1042/bj0950393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHAPPELL J. B., GREVILLE G. D. Effects of oligomycin on respiration and swelling of isolated liver mitochondria. Nature. 1961 May 6;190:502–504. doi: 10.1038/190502a0. [DOI] [PubMed] [Google Scholar]
  5. CHAPPELL J. B., GREVILLE G. D. Inhibition of electron transport and the swelling of isolated mitochondria. Nature. 1959 May 30;183(4674):1525–1526. doi: 10.1038/1831525a0. [DOI] [PubMed] [Google Scholar]
  6. CHAPPELL J. B., GREVILLE G. D. Mitochondrial swelling and electron transport. I. Swelling supported by ferricyanide. Biochim Biophys Acta. 1960 Mar 11;38:483–494. doi: 10.1016/0006-3002(60)91283-x. [DOI] [PubMed] [Google Scholar]
  7. CHAPPELL J. B. The effect of alkylguanidines on mitochondrial metabolism. J Biol Chem. 1963 Jan;238:410–417. [PubMed] [Google Scholar]
  8. CROFTS A. R., CHAPPELL J. B. CALCIUM ION ACCUMULATION AND VOLUME CHANGES OF ISOLATED LIVER MITOCHONDRIA. REVERSAL OF CALCIUM ION-INDUCED SWELLING. Biochem J. 1965 May;95:387–392. doi: 10.1042/bj0950387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chappell J. B. The effects of 2,4-dinitrophenol on mitochondrial oxidations. Biochem J. 1964 Feb;90(2):237–248. doi: 10.1042/bj0900237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chappell J. B. The oxidation of citrate, isocitrate and cis-aconitate by isolated mitochondria. Biochem J. 1964 Feb;90(2):225–237. doi: 10.1042/bj0900225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ERNSTER L., JALLING O., LOW H., LINDBERG O. Alternative pathways of mitochondrial DPNH oxidation, studied with amytal. Exp Cell Res. 1955;(Suppl 3):124–132. [PubMed] [Google Scholar]
  12. ESNOUF M. P., WILLIAMS W. J. The isolation and purification of a bovine-plasma protein which is a substrate for the coagulant fraction of Russell's-viper venom. Biochem J. 1962 Jul;84:62–71. doi: 10.1042/bj0840062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gutfreund H., Jones E. A. The kinetic behaviour of enzymes in organized systems. Mitochondrial succinate oxidase and fumarase. Biochem J. 1964 Jan;90(1):208–213. doi: 10.1042/bj0900208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HEYTLER P. G., PRICHARD W. W. A new class of uncoupling agents--carbonyl cyanide phenylhydrazones. Biochem Biophys Res Commun. 1962 May 4;7:272–275. doi: 10.1016/0006-291x(62)90189-4. [DOI] [PubMed] [Google Scholar]
  15. HUNTER F. E., Jr, FORD L. Inactivation of oxidative and phosphorylative systems in mitochondria by preincubation with phosphate and other ions. J Biol Chem. 1955 Sep;216(1):357–369. [PubMed] [Google Scholar]
  16. LEHNINGER A. L., REMMERT L. F. An endogenous uncoupling and swelling agent in liver mitochondria and its enzymic formation. J Biol Chem. 1959 Sep;234:2459–2464. [PubMed] [Google Scholar]
  17. LEHNINGER A. L., ROSSI C. S., GREENAWALT J. W. Respiration-dependent accumulation of inorganic phosphate and Ca ions by rat liver mitochondria. Biochem Biophys Res Commun. 1963 Mar 25;10:444–448. doi: 10.1016/0006-291x(63)90377-2. [DOI] [PubMed] [Google Scholar]
  18. LINDAHL P. E., OBERG K. E. The effect of rotenone on respiration and its point of attack. Exp Cell Res. 1961 Mar;23:228–237. doi: 10.1016/0014-4827(61)90033-7. [DOI] [PubMed] [Google Scholar]
  19. LONGMUIR I. S. Respiration rate of rat-liver cells at low oxygen concentrations. Biochem J. 1957 Feb;65(2):378–382. doi: 10.1042/bj0650378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PACKER L. Metabolic and structural states of mitochondria. I. Regulation by adenosine diphosphate. J Biol Chem. 1960 Jan;235:242–249. [PubMed] [Google Scholar]
  21. RAAFLAUB J. Die Schwellung isolierter Leberzellmitochondrien und ihre physikalisch-chemische Beeinflussbarkeit. Helv Physiol Pharmacol Acta. 1953;11(2):142–156. [PubMed] [Google Scholar]
  22. SLATER E. C., CLELAND K. W. The effect of tonicity of the medium on the respiratory and phosphorylative activity of heart-muscle sarcosomes. Biochem J. 1953 Mar;53(4):557–567. doi: 10.1042/bj0530557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. TAPLEY D. F. The effect of thyroxine and other substances on the swelling of isolated rat liver mitochondria. J Biol Chem. 1956 Sep;222(1):325–339. [PubMed] [Google Scholar]
  24. WOJTCZAK L., LEHNINGER A. L. Formation and disappearance of an endogenous uncoupling factor during swelling and contraction of mitochondria. Biochim Biophys Acta. 1961 Aug 19;51:442–456. doi: 10.1016/0006-3002(61)90600-x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES