Abstract
The frequencies of recovered disomy among the meiotic segregants of yeast (Saccharomyces cerevisiae) triploids were assessed under conditions in which all 17 yeast chromosomes were monitored simultaneously. The studies employed inbred triploids, in which all homologous centromeres were identical by descent, and single haploid testers carrying genetic markers for all 17 linkage groups. The principal results include: (1) Ascospores from triploid meiosis germinate at frequencies comparable to those from normal diploids, but most fail to produce visible colonies due to the growth-retarding effects of high multiple disomy. (2) The probability of disome formation during triploid meiosis is the same for all chromosomes; disomy for any given chromosome does not exclude simultaneous disomy for any other chromosome. (3) The 17 yeast chromosomes fall into three frequency classes in terms of disome recovery. The results support the idea that multiply disomic meiotic segregants of the triploid experience repeated, nonrandom, post-germination mitotic chromosome losses (N+1→N) and that the observed variations in individual disome recovery are wholly attributable to inherent differences in disome mitotic stability.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Byers B., Shriver K., Goetsch L. The role of spindle pole bodies and modified microtubule ends in the initiation of microtubule assembly in Saccharomyces cerevisiae. J Cell Sci. 1978 Apr;30:331–352. doi: 10.1242/jcs.30.1.331. [DOI] [PubMed] [Google Scholar]
- Campbell D. Association of disomic chromosome loss with EMS-induced conversion in yeast. Genetics. 1980 Nov;96(3):613–625. doi: 10.1093/genetics/96.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Culbertson M. R., Henry S. A. Genetic analysis of hybrid strains trisomic for the chromosome containing a fatty acid synthetase gene complex (fas1) in yeast. Genetics. 1973 Nov;75(3):441–458. doi: 10.1093/genetics/75.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fogel S., Roth R. Mutations affecting meiotic gene conversion in yeast. Mol Gen Genet. 1974 May 31;130(3):189–201. doi: 10.1007/BF00268799. [DOI] [PubMed] [Google Scholar]
- Hilger F., Mortimer R. K. Genetic mapping of arg1 and arg8 in Saccharomyces cerevisiae by trisomic analysis combined with interallelic complementation. J Bacteriol. 1980 Jan;141(1):270–274. doi: 10.1128/jb.141.1.270-274.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rodarte-Ramón U. S., Mortimer R. K. Radiation-induced recombination in Saccharomyces: isolation and genetic study of recombination-deficient mutants. Radiat Res. 1972 Jan;49(1):133–147. [PubMed] [Google Scholar]
- Roth R., Fogel S. A system selective for yeast mutants deficient in meiotic recombination. Mol Gen Genet. 1971;112(4):295–305. doi: 10.1007/BF00334431. [DOI] [PubMed] [Google Scholar]
- Wickner R. B. Mapping chromosomal genes of Saccharomyces cerevisiae using an improved genetic mapping method. Genetics. 1979 Jul;92(3):803–821. doi: 10.1093/genetics/92.3.803. [DOI] [PMC free article] [PubMed] [Google Scholar]