Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1981 Jul;98(3):491–503. doi: 10.1093/genetics/98.3.491

Inositol Mutants of SACCHAROMYCES CEREVISIAE: Mapping the ino1 Locus and Characterizing Alleles of the ino1, ino2 and ino4 Loci

Thomas F Donahue 1,2, Susan A Henry 1,2
PMCID: PMC1214455  PMID: 17249096

Abstract

An extensive genetic analysis of inositol auxotrophic mutants of yeast is reported. The analysis includes newly isolated mutants, as well as those previously reported (Culbertson and Henry 1975). Approximately 70% of all inositol auxotrophs isolated are shown to be alleles of the ino1 locus, the structural gene for inositol-1-phosphate synthase, the major enzyme involved in inositol biosynthesis. Alleles of two other loci, ino2 and ino4, comprise 9% of total mutants, with the remainder representing unique loci or complementation groups. The ino1 locus was mapped by trisomic analysis with an n + 1 disomic strain constructed with complementing alleles at this locus. The ino1 locus is shown to be located between ura2 (11.1 cm) and cdc6 (21.8 cm) on chromosome X. An extended map of chromosome X of yeast is presented. Unlike most yeast loci, but similar to the his1 locus, the ino1 locus lacks allelic representatives that are suppressible by known suppressors. This finding suggests that premature termination of translation of the ino1 gene product may be incompatible with cell viability.

Full Text

The Full Text of this article is available as a PDF (832.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FINK G. R. GENE-ENZYME RELATIONS IN HISTIDINE BIOSYNTHESIS IN YEAST. Science. 1964 Oct 23;146(3643):525–527. doi: 10.1126/science.146.3643.525. [DOI] [PubMed] [Google Scholar]
  2. Fogel S., Lax C., Hurst D. D. Reversion at the HiS1 locus of yeast. Genetics. 1978 Nov;90(3):489–500. doi: 10.1093/genetics/90.3.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hawthorne D. C., Mortimer R. K. Genetic mapping of nonsense suppressors in yeast. Genetics. 1968 Dec;60(4):735–742. doi: 10.1093/genetics/60.4.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lawrence C. W., Sherman F., Jackson M., Gilmore R. A. Mapping and gene conversion studies with the structural gene for iso-1-cytochrome C in yeast. Genetics. 1975 Dec;81(4):615–629. doi: 10.1093/genetics/81.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lax C., Fogel S. Novel interallelic complementation at the his1 locus of yeast. Genetics. 1978 Nov;90(3):501–516. doi: 10.1093/genetics/90.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Liebman S. W., Stewart J. W., Parker J. H., Sherman F. Leucine insertion caused by a yeast amber suppressor. J Mol Biol. 1977 Jan 5;109(1):13–22. doi: 10.1016/s0022-2836(77)80043-0. [DOI] [PubMed] [Google Scholar]
  7. Mortimer R. K., Hawthorne D. C. Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes. Genetics. 1973 May;74(1):33–54. doi: 10.1093/genetics/74.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Perkins D. D. Biochemical Mutants in the Smut Fungus Ustilago Maydis. Genetics. 1949 Sep;34(5):607–626. doi: 10.1093/genetics/34.5.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Shaffer B., Brearley I., Littlewood R., Fink G. R. A stable aneuploid of Saccharomyces cerevisiae. Genetics. 1971 Apr;67(4):483–495. doi: 10.1093/genetics/67.4.483. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES